【】? Objective? The preparation of an animal model of asthenozoospermia with hypersperm DNA fragmentation was explored by inducing the construction of a mouse model of asthenozoospermia (AZS) with hypersperm DNA fragmentation (SDF) by the hormone corticosterone (CORT) and the compound sodium benzoate (NaB).? Methods? Fifty 3-week-old male ICR mice were randomly divided into 30 CORT-treated groups and 20 NaB-treated groups. The CORT treatment group was divided into 6 groups, with 5 animals in each group, of which 3 groups were high (500 μg/ml), medium (200 μg/ml) and low (10 μg/ml) dose groups of CORT drinking water modeling, 1 group was drinking water control group, 1 group was CORT injection molding group (40 mg/kg), and 1 group was injection control group (normal saline), and the model was continuously modeled for 50 days. The NaB treatment group was divided into 4 groups, with 5 animals in each group, of which 3 groups were high (500 mg/kg), medium (300 mg/kg) and low (100 mg/kg) NaB gavage groups, and 1 group was control group (normal saline), and the model was continuously modeled for 50 days. The physiological state of the mice in each group was observed and the weight changes were continuously recorded, and the sperm motility capacity and DNA fragmentation index (DFI) of the sperm were observed from the tail of the epididymis of the mice after the end of the modeling. Results? The rate of weight change in the CORT injection group showed a downward trend, There was no significant difference (P>0.05) in the high-dose NaB gavage group, and the change rate of body weight in the high-dose NaB gavage group decreased significantly compared with the control group (P<0.05), the percentage of forward motility sperm in the CORT injection group decreased compared with the control group (P>0.05), the percentage of forward motility sperm in the high-dose NaB gavage group decreased significantly compared with the control group (P<0.05), the DFI in the CORT injection group increased with no significant difference compared with the control group (P>0.05), and the DFI in the high-dose NaB gavage group increased significantly compared with the control group (P>0.05).? Conclusion? Intragastric gavage at NaB 500mg/(kg·d) for 50 days is an ideal method to construct an animal model of AZS with high SDF.