Berberine is a natural Isoquinoline alkaloid, which can be initially used as a broad-spectrum antibacterial agent in clinical treatment of enteritis, peptic ulcer, chronic gastritis, pneumonia and other diseases. In recent years, with the in-depth study of the pharmacological effects of Berberine, more and more evidence shows that Berberine has neuroprotective effects on ischemic stroke. In this review, we introduced the intervention of Berberine on risk factors of ischemic stroke, and discussed the neuroprotective effects of Berberine in different mechanisms of ischemic stroke in detail, aiming to provide some reference for clinical and basic research in this field.