Objective: To study the effect of mongolian drugs Hatagaqi-7 promoting ulcer wound healing of diabetes rats via hypoxia inducible factor-1α(HIF-1α). Methods: Adult male SD rats were randomly divided into control group, diabetes group, mongolian drug group and cytokine group. Besides the control group, the other three groups were treated with intraperitoneal injection of streptozotocin to establish the diabetes model. The ulcer wounds were prepared in the back of the four groups. One week later, the mongolian drug group was treated with hattagelge-7, and the cytokine group was treated with recombinant bovine basic fibroblast growth factor for two consecutive weeks. Then fasting blood glucose (FBG), wound area, wound pathology, the expression level of advanced glycation end products (AGEs), receptor of AGE (RAGE), HIF-1α, vascular endothelial growth factor (VEGF) and the levels of interleukin-1β(IL-1β), interferon-γ(IFN-γ), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) were detected. Results: The FBG of diabetes group, mongolian drug group and cytokine group were higher than those of the the control group(P<0.05), and there was no significant difference among the three groups (P>0.05). Compared with the control group, the area of ulcer wound, the scope of unrepaired tissue under microscope, the expression level of AGEs, RAGE and the levels of IL-1β, IFN-γ, MDA in the wound tissue of the diabetes group increased, the level of T-AOC and expression levels of HIF-1α, VEGF of the diabetes group decreased (P<0.05). Compared with the diabetes group, the area of ulcer wound, the scope of unrepaired tissue under microscope, the expression level of AGEs, RAGE and the levels of IL-1β, IFN-γ, MDA in the wound tissue of the mongolian drug group and cytokine group decreased, the level of T-AOC and expression levels of HIF-1α, VEGF of the mongolian drug group and cytokine group increased (P<0.05) and the indexes of mongolian drug group were better than those of cytokine group. Conclusion: Mongolian drugs Hatagaqi-7 promotes ulcer wound healing of diabetes rats, and the inhibiton of AGEs and RAGE expression and the activaton of HIF-1 α is a possible molecular mechanism.