This review introduces the daily management practices related to laboratory animal licensing in Hubei Province and the supervision processes during and after licensing. We consider the current status of laboratory animal license management, achievements, problems, and countermeasures in Hubei Province, with a focus on analyzing the legislative situation for laboratory animals, the issuance and distribution of permits, the scale of facilities, and the composition of employees. The number of laboratory animal licenses issued in Hubei Province has recently been increasing year by year, and the numbers of animals produced and used by licensed units have also continued to rise. Although the related industries are flourishing however, there are some regulatory deficiencies. This paper considers the perspective of biosafety, combined with the problems encountered in license management, and proposes relevant safety supervision countermeasures and suggestions to promote the development of the laboratory animal industry in Hubei Province.