BEI Xueyi
1. Hunan University of Chinese Medicine, Changsha 410208, China. 2. Hunan Engineering and Technology Research Center of Medicinal and Edible Homologous Functional Food, Hunan University of Chinese Medicine,Changsha 410208. 3. Key Laboratory of TCM Cardiopulmonary Syndrome Differentiation and Medicinal Diet Diet Therapy, Hunan University of Chinese Medicine, Changsha 410208JIANG Ning
4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193YAO Caihong
4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193ZHANG Yiwen
4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193SUN Xinran
4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193LUO Yanqin
4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193LI Liang
1. Hunan University of Chinese Medicine, Changsha 410208, China. 2. Hunan Engineering and Technology Research Center of Medicinal and Edible Homologous Functional Food, Hunan University of Chinese Medicine,Changsha 410208. 3. Key Laboratory of TCM Cardiopulmonary Syndrome Differentiation and Medicinal Diet Diet Therapy, Hunan University of Chinese Medicine, Changsha 410208XIE Mengzhou
1. Hunan University of Chinese Medicine, Changsha 410208, China. 2. Hunan Engineering and Technology Research Center of Medicinal and Edible Homologous Functional Food, Hunan University of Chinese Medicine,Changsha 410208. 3. Key Laboratory of TCM Cardiopulmonary Syndrome Differentiation and Medicinal Diet Diet Therapy, Hunan University of Chinese Medicine, Changsha 410208LIU Xinmin
5. Institute of New Drug Technology, Ningbo University, Ningbo 3150001. Hunan University of Chinese Medicine, Changsha 410208, China. 2. Hunan Engineering and Technology Research Center of Medicinal and Edible Homologous Functional Food, Hunan University of Chinese Medicine,Changsha 410208. 3. Key Laboratory of TCM Cardiopulmonary Syndrome Differentiation and Medicinal Diet Diet Therapy, Hunan University of Chinese Medicine, Changsha 410208. 4. Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193. 5. Institute of New Drug Technology, Ningbo University, Ningbo 315000
R-33
Objective To compare the effects of ginsenosides Rg1 and Rb1 on depression- and anxiety-like behaviors in chronic unpredictable stress-induced rats. Methods Seventy male SPF grade SD rats were tested for sugar and water preference after 5 days of adaptation and divided into seven groups according to their preference index: a control group, model group, fluoxetine hydrochloride group, ginsenoside Rg1 24 mg / kg group, ginsenoside Rg1 48 mg / kg group,ginsenoside Rb1 33 mg / kg group, and ginsenoside Rb1 67 mg / kg group. All rats, except for the control group, were subjected randomly to one or two different stimulating factors every day for a total of 35 days. On the 36th day, behavioral experiments including sugar and water preference, open field, novel environment feeding inhibition, elevated cross maze,and forced swimming experiments were conducted to investigate the anti-depression and anti-anxiety effects of the treatments. Serum and hippocampal levels of interleukin ( IL)-1β, IL-6, tumor necrosis factor ( TNF)-α and serum corticosterone were measured by enzyme-linked immunosorbent assay. Results Compared with the model group, ginsenoside Rg1 and Rb1 significantly increased sucrose consumption in the sucrose preference test and decreased immobility in the forced swimming test. Ginsenoside Rg1 ( 48 mg / kg) significantly reduced the latency to eat in the novelty-suppressed feeding test, and ginsenoside Rg1 (24 and 48 mg / kg) significantly increased the percentage of open arm entries and time in the elevated cross maze test. Serum corticosterone levels were significantly decreased in the ginsenoside Rg1 and Rb1 groups, serum IL-1β and IL-6 levels were significantly decreased in the ginsenoside Rg1 ( 48mg / kg) group, serum TNF-α and IL-6 levels were significantly decreased in the ginsenoside Rb1 (33 mg / kg) group, and IL-1β, IL-6, and TNF-α levels in the hippocampus were significantly decreased in the ginsenoside Rg1 (48 mg / kg) and Rb1 (67 mg / kg) groups. Conclusions Both ginsenosides can regulate the hypothalamic-pituitary-adrenal axis and inhibit neuroinflammation, improving depression- and anxiety-like behaviors in rats induced by chronic unpredictable stress. Ginsenoside Rg1 has a significantly better anti-anxiety effect than Rb1.
