Progress in the optimization of CRISPR / Cas9 technology for the establishment of genetically modified mouse models

doi:10.3969/j.issn.1671-7856.2024.05.017

Home > Archive>Volume 34, Issue 5, 2024 >-152. DOI:10.3969/j.issn.1671-7856.2024.05.017

Progress in the optimization of CRISPR / Cas9 technology for the establishment of genetically modified mouse models
DOI:
                        10.3969/j.issn.1671-7856.2024.05.017
                    
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Author:
                        WANG YuWANG Yu
Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC),Beijing 102629, China
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WU YongWU Yong
Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC),Beijing 102629, China
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LIANG ChunnanLIANG Chunnan
Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC),Beijing 102629, China
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FAN ChangfaFAN Changfa
Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC),Beijing 102629, China
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Affiliation:Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC),Beijing 102629, China
Clc Number:R-33
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Abstract:

CRISPR/ Cas9 technology has driven the development of various fields in life science. With continuous deepening of its understanding, researchers have made multiple improvements and optimizations to adapt to various application scenarios. The optimization of CRISPR/ Cas9 technology has also provided breakthroughs in the establishment of genetically modified mouse models. This article briefly reviews the development process of CRISPR/ Cas9 technology and summarizes optimization strategies of CRISPR/ Cas9, establishment of conditional knockout / knockin gene-modified mouse models, and delivery systems for CRISPR/ Cas9 elements and HDR templates.

Key words:CRISPR/ Cas9; mouse model; conditional knockout / knockin; delivery

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Received:July 10,2023
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Online: June 26,2024
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