Objective To study the protective effect of an external medicine “hemorrhoid magnetized cream” on acetic acid-induced hemorrhoids in mice. Methods Forty SPF ICR mice were divided into normal (n =10) and model (n =30) groups. The model was established with 20% acetic acid and divided into untreated model, hemorrhoid magnetized cream-treated and Ma Yinglong musk hemorrhoid cream-treated groups at 24 hours after hemorrhoid induction. After 11 d of treatment, the perianal scores were determined, levels of L-6, IL-1β and TNF-α in peripheral blood were detected by CBA kit, nitric oxide (NO) in peripheral blood was detected by Griess kit, and hematoxylin-eosin (HE) staining was performed to evaluate the pathological changes in rectal tissue. Results Compared with the normal group, the perianal score of the untreated model group was significantly increased, the pathological damage to the rectum was severe, and the levels of IL- 6, IL-1β, TNF-α and NO in the peripheral blood were significantly increased. Compared with the untreated model group, the perianal scores in the hemorrhoid magnetized cream- and Ma Yinglong musk hemorrhoid cream-treated groups were significantly reduced, the pathological changes in the rectal tissue tissues were significantly improved, and the levels of IL- 6, IL-1β, TNF-α and NO in peripheral blood were significantly decreased. Conclusions The hemorrhoid magnetized cream shows a good therapeutic effect in the mouse hemorrhoid model, and this therapeutic effect is closely related to the regulation of inflammation of the body.