Objective To study the rapid diabetes modeling with KK feed and its effect on related indexes in KK/Upj-A^Y / J mice. Methods Male and female (40 each) KK/ Upj-A^Y / J mice were divided into four groups: common feedgroup (male and female each 10), KK feed group (male and female each 10), fasting blood glucose group fed commonfeed (male and female each 10), and fasting blood glucose group fed KK feed (male and female each 10). The mice werefed from the age of 3 weeks to 23 weeks. Weight and fasting blood glucose values were measured each week. At the end ofthe experiment serum and organ tissue samples were collected for biochemical analysis, pathological examination using HEand special stainings were used for pathological analysis, the pancreas tissues was stained using an insulin IHC method ,and the liver with PAS and the kidney with PASM staining. Results KK feed significantly promoted the body weight gain,increased the fasting blood glucose, and the serum ALT content ( P < 0. 05). In the KK feed group, the CRE level infemale mice and the CHO level in male mice were significantly increased ( P < 0. 05), however, there was no significanteffect on the CHO level in female mice. At the end of the feeding experiment, there were obvious changes in the pancreaticpathology in female mice, but there was no significant change in males. KK Feed significantly promoted steatosis in livercells from the KK/ Upj-A^Y / J mice. Compared with the C57BL/6 J mice, the renal pathological changes in KK/ Upj-A^Y / Jmice at the end of the experiment were mainly tubular type, interstitial glomerulonephritis and perivascular inflammation.The glomerular mesangial matrix was significantly increased in the KK/ Upj-A^Y / J mice. The degree of renal lesion was moreobvious in the KK feed group. Compared with the control group (C57BL/6 J mice), the renal PASM staining showed thatthe glomerular basement membrane in the other two groups had no dark brown substance deposition, indicating thedestruction of the glomerular basement membrane structure. Conclusions KK feed promotes diabetic organ injury,increases the fasting blood glucose value and body weight, shortens the modeling time and improves the uniformity of modeling in KK/ Upj-A^Y / J mice.