Comparative Medicine Center of Yangzhou University
目的 探究纳米二氧化铈（CeO2NPs）亚急性腹腔染毒对ICR小鼠体重、主要脏器以及血清氧化还原指标的影响。方法 将雄性ICR小鼠35只随机分成5组，每组7只。设生理盐水对照组和不同浓度的CeO2NPs（100、500、1000、5000 μg/kg）试验组。小鼠腹腔隔日注射CeO2NPs，28 d后进行相关指标的检测。结果 与对照组相比，CeO2NPs各剂量组小鼠体重增长差异不显著（P>0.05）；5000 ug/kg剂量组肝脏系数、附睾系数与精子存活率与对照组相比差异极显著（P<0.01）。5000 ug/kg剂量组肝脏和脾脏内可见纳米氧化铈沉积，部分肝脏中可见肉芽肿。100 ug/kg剂量组血清SOD和GSH-Px活力升高，MDA含量降低，体现出CeO2NPs的抗氧化作用，随着注射剂量的升高，CeO2NPs的氧化应激作用增强。结论 低剂量CeO2NPs对机体较安全，并表现出一定的抗氧化作用，随着剂量增加，CeO2NPs对机体的毒性也逐渐增强。
Objective To investigate the effects of CeO2NPs sub-acute exposure on body weight, organ structures and redox indicators in male mice. Methods 35 male ICR mice were randomly divided into five groups with seven mice in each group. Normal control group and CeO2NPs (100, 500, 1000, 5000 ug/kg) test groups were set up. Mice were injected CeO2NPs intraperitoneally every other day for 28 days. Results Compared with the control group, there was no significant difference in weight gain (P>0.05) , but there was significant difference in liver coefficient, epididymal coefficient and sperm survival rate (P<0.01). In the 5000 ug/kg group, CeO2NPs were deposited in the liver and spleen, and granuloma was found. The activities of SOD and GSH-Px in serum of 100 ug/kg group were increased, while the content of MDA was decreased, which showed the antioxidant effect of CeO2NPs. With the increase of dose, the oxidative stress induced by CeO2NPs was enhanced. Conclusion The low dose of CeO2NPs is safe and has antioxidant effect. With the increase of dose, the toxicity of CeO2NPs to the body also increases gradually.