基于THP-1细胞的皮肤致敏体外检测方法的研究
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广东省科技计划项目


The Study of In Vitro Skin Sensitization Test Based on THP-1 Cell Line
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Supported燽y燬cience燼nd燭echnology燩lanning燩roject爋f燝uangdong燩rovince,燙hina

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    摘要:

    【摘要】: 目的 建立基于人细胞系的替代皮肤致敏动物实验的体外检测方法(h-CLAT),并对化学品、日用化学产品和化妆品植物原料进行皮肤致敏性检测。方法 体外培养人急性单核细胞白血病细胞(THP-1),不同浓度受试物与细胞共孵育24h,通过流式细胞仪检测细胞表面标志物CD86和CD54活化的差异,并对11种已知皮肤致敏化学物质和9种未知样品进行皮肤致敏性预测。同时对未知样品进行豚鼠局部封闭涂皮法验证。结果 使用h-CLAT方法准确区分了11种参考物质的皮肤致敏性,9种受试样品中,7种样品被判断为阴性皮肤致敏物质,2种植物提取物被确认为皮肤致敏疑似物质。9种样品的预测结果与动物实验一致。结论 h-CLAT体外检测方法可以代替部分动物测试,用于可溶性皮肤致敏物质的预测。

    Abstract:

    【Abstract】: Objective Construction of h-CLAT testing method based on THP-1 cell line to build a assessment plans for skin sensitization powerful potency of chemicals, personal care products and plant extract. Method THP-1 cells was cultured in vitro and exposed to substances that were 11 reference skin sensitization chemicals and 9 samples,by monitoring the cell viability, cell surface marker CD54 /CD86 and relative fluorescence intensity of cells surface after the cells was exposures substances, to discover whether positive. Simultaneously, Buehler test was used to validate the results from h-CLAT. Results 11 reference chemicals were distinguished correctly by h-CLAT, subsequently, the 7 samples were recognized negative and 2 samples were perceived positive. The qualitative classification of sensitization was corresponded to the results of h-CLAT in 9 samples. Conclusion the h-CLAT-in vitro test methods can be used to replace some of the animal tests for the prediction of soluble skin allergy

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陈彧.基于THP-1细胞的皮肤致敏体外检测方法的研究[J].中国比较医学杂志,2017,27().

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  • 收稿日期:2016-07-04
  • 最后修改日期:2016-07-31
  • 录用日期:2016-12-01
  • 在线发布日期: 2018-08-16
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