Abstract:【Abstract】: Objective Construction of h-CLAT testing method based on THP-1 cell line to build a assessment plans for skin sensitization powerful potency of chemicals, personal care products and plant extract. Method THP-1 cells was cultured in vitro and exposed to substances that were 11 reference skin sensitization chemicals and 9 samples,by monitoring the cell viability, cell surface marker CD54 /CD86 and relative fluorescence intensity of cells surface after the cells was exposures substances, to discover whether positive. Simultaneously, Buehler test was used to validate the results from h-CLAT. Results 11 reference chemicals were distinguished correctly by h-CLAT, subsequently, the 7 samples were recognized negative and 2 samples were perceived positive. The qualitative classification of sensitization was corresponded to the results of h-CLAT in 9 samples. Conclusion the h-CLAT-in vitro test methods can be used to replace some of the animal tests for the prediction of soluble skin allergy