目的 研究D-半乳糖对成年小鼠海马神经元P—ERK 1／2表达和学习记忆能力的影响。方法 20只健康成年昆明种小鼠，雌雄各半，分为2组。颈背部皮下注射D-半乳糖注射液，每天125mg/kg bw，连续35天，建立小鼠衰老模型。对照组注射生理盐水。其间观察小鼠一般状况，实验的最后1W分别进行跳台实验、Morris水迷宫实验检测小鼠的学习记忆功能；实验结束后处死动物，提取海马组织总蛋白，免疫印迹检测P—ERK1／2表达。结果 与对照组比较，衰老模型组小鼠跳台试验潜伏时间减少和电击次数增加（P<0.01）；Morris水迷宫实验航行逃避潜伏期明显大于对照组小鼠（p<0.01）；模型组P-ERK1/2含量显著低于正常对照组（P<0.01），且雄性比雌性效果显著。结论 D-半乳糖可能通过抑制成年小鼠海马神经元P—ERK1／2表达而损害其学习记忆能力，且雄性小鼠效果更明显。
[Abstract] Objective To study the effects of D-galactose on the expression of P-ERK 1/2 in hippocampus of mice and on learning and memory ability Methods Twenty Kunming mice, half male and half female, were divided into two groups. To establish aging model of mice, D-galactose was injected daily in the subcutaneous for 35 days in the dosage of 125 mg / kg. The control group was given saline. In the modeling period, the general condition of mice was observed. Step-down and morris water maze were carried out to test the learning and memory ability at the last one week. After the experiment, the animals were sacrificed to extract hippocampal tissue. The expression of P-ERK1 / 2 in hippocampal of mice was detected by western blot. Results Step-down test showed that compared with the control group, the latency in the model group decrease significantly (P <0.01) and the number of electric shock in the model group increase significantly (P <0.01). Morris water maze showed that the latency of escape in the model group were longer than the control group (P <0.01). Western blotting results showed that the expression of P-ERK1 / 2 significantly decreased in the model group and the effect is more pronounced in male mice. Conclusion D-galactose may impair learning and memory ability by inhibiting the expression of P-ERK1 / 2 in hippocampal of mice and the effect is more pronounced in male mice.