西方饮食饲料对 APOE- / - 小鼠动脉粥样硬化造模的影响
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北京华阜康生物科技股份有限公司,北京 102202

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R-33


Effect of Western diet on APOE- / - atherosclerosis model mice
Author:
Affiliation:

Beijing HFK Bioscience CO. , LTD, Beijing 102202, China

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    摘要:

    目的 为了研究西方饮食饲料快速建模及对 APOE- / - 小鼠生物学指标及组织病理学的影响。 方法用 48♀ 48♂APOE- / - 小鼠、48♀ 48♂C57BL/ 6J 进行了试验,分为 8 组,分别为 APOE- / - 普通繁殖料组(24♀ 24♂)和 APOE- / - -西方饮食饲料组(24♀ 24♂)、C57BL/ 6J 普通繁殖料组(24♀ 24♂)和 C57BL/ 6J 西方饮食饲料组(24♀24♂)。 从 3 周开始饲喂,直到 20 周实验结束。 实验结束后采集血清检测生化指标,分离主动脉做大体油红 O 染色及分析,主动脉根部做石蜡切片和 HE 染色。 结果 西方饮食没有显著增加 APOE- / - 体重,但可以显著提高APOE- / - 鼠的血脂指标、总胆固醇、低密度脂蛋白、高密度脂蛋白,促进动脉粥样硬化斑块的形成,雄鼠适合主动脉大体斑块的造模,雌鼠适合主动脉弓根部斑块造模。 结论 西方饮食饲料可以促进 APOE- / - 小鼠动脉粥样硬化造模,增加主动脉斑块面积比,缩短建模时间,提高建模的均一性。

    Abstract:

    Objective To study the impact of a Western diet-type feed on biological indicators and histopathology in APOE- / - mice. Methods Forty-eight female and 48 male APOE- / - mice, and 48 female and 48 male C57BL/ 6J mice were divided into eight breeding groups: APOE- / - breeding feed group, APOE- / - Western dietary feed group, C57BL/ 6J breeding feed group, and C57BL/ 6J Western dietary feed group (24 male and 24 female mice per group). Mice were fed the respective diets from 3 weeks until the end of the experiment at 20 weeks. After the experiment, serum was collected for measurement of biochemical indicators. Aortas were removed for oil red O staining and gross examination and the aorta root was paraffin sectioned and stained with hematoxylin and eosin. Results A Western diet did not significantly increase body weight in APOE- / - mice, but did significantly improve the blood lipid index and total cholesterol, low-density lipoprotein,and high-density lipoprotein levels, and promoted the formation of atherosclerotic plaques. Male mice were suitable for modeling gross aortic plaques while female mice were suitable for modeling aortic arch root plaques. Conclusions A Western diet can promote atherosclerosis in APOE- / - mice, increase the aortic plaque area ratio, shorten modeling time,and improve modeling uniformity.

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王 蕾,宋辉欠,李 斌,梁 超,陈 敏,田玉淑,吴旭颖,张文明,刘云波.西方饮食饲料对 APOE- / - 小鼠动脉粥样硬化造模的影响[J].中国比较医学杂志,2024,34(7):29~38.

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  • 收稿日期:2023-07-31
  • 在线发布日期: 2024-08-19
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