中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
R-33
LIU Qi
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaHOU Yongzhi
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaLI Na
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaZHANG Jingjing
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaLU Jiahan
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaCONG Zhe
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaZHU Lin
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaXUE Jing
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaInstitute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
目的 探索 CAST/ EiJ 小鼠对多种病原体易感的可能原因,分析 CAST/ EiJ 小鼠外周血和脾内的免疫细胞表型,明确 CAST/ EiJ 小鼠的免疫细胞构成。 方法 利用流式细胞术,通过细胞表面标记物 CD3、CD4、CD8、CD11b、CD11c、CD19、CD27、CD49b 和 TCRβ 检测 CAST/ EiJ 小鼠和 C57BL/ 6J 小鼠外周血和脾内经典型树突状细胞(cDCs)、自然杀伤细胞(NK)、B 淋巴细胞、T 淋巴细胞及其亚群的细胞比例。 结果 CAST/ EiJ 小鼠与 C57BL/ 6J 小鼠的 cDCs 细胞比例无明显差异,但 cDC1 细胞亚群比例偏低;CAST/ EiJ 小鼠的 NK 细胞(主要是成熟 NK 细胞亚群)和 T 淋巴细胞(主要是 CD8+T 细胞)比例都显著低于 C57BL/ 6J 小鼠,而 CAST/ EiJ 小鼠的 B 淋巴细胞比例高于C57BL/ 6J 小鼠。 结论 CAST/ EiJ 小鼠中 NK 和 T 淋巴细胞比例显著低于 C57BL/ 6J 小鼠。
Objective To explore the possible causes of CAST/ EiJ mouse susceptibility to multiple pathogens,the immune cell phenotypes in the peripheral blood and spleen of CAST/ EiJ mice were analyzed to clarify their composition. Methods Classical dendritic cells (cDCs), natural killer (NK) cells, B lymphocytes, T lymphocytes, and their subsets in the peripheral blood and spleen of CAST/ EiJ mice and C57BL/ 6J mice were detected by flow cytometry using the cell surface markers CD3, CD4, CD8, CD11b, CD11c, CD19, CD27, CD49b, and TCRβ. Results There was no significant difference in the proportion of cDCs between CAST/ EiJ and C57BL/ 6J mice, but the cDC1 cell subset population was smaller in CAST/ EiJ. The proportions of NK cells ( mainly mature NK cell subsets) and T lymphocytes(mainly CD88+T cells) were both lower in CAST/ EiJ mice than C57BL/ 6J mice, while the proportion of B cells was higher in CAST/ EiJ mice than C57BL/ 6J mice. Conclusions The proportions of NK and T lymphocytes in CAST/ EiJ mice were lower than those in C57BL/ 6J mice.
刘 琪,侯勇志,李 娜,张京京,陆佳涵,丛 喆,朱 林,薛 婧. CAST / EiJ 小鼠外周血和脾中免疫细胞及亚群分析[J].中国比较医学杂志,2024,34(4):1~10.
复制