1.成都中医药大学,成都 611137;2.中南大学湘雅二医院,长沙 410013;3.彭州市人民医院,四川 彭州 611930;4.川北医学院附属医院,四川 南充 637000
R-33
1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. 2. the Second Xiangya Hospital of Central South University, Changsha 410013. 3. the People’s Hospital of Pengzhou, Pengzhou 611930. 4. Affiliated Hospital of North Sichuan Medical College, Nanchong 637000
目的 探讨甘草泻心汤对溃疡性结肠炎(UC)大鼠水通道蛋白3、4 的影响。 方法 清洁级SD 大鼠32 只,抽取8 只作为空白组,余下24 只采用4%葡聚糖硫酸钠复制UC 模型,模型成功后随机分为模型组,西药组(美沙拉嗪缓释颗粒),中药组(甘草泻心汤),每组8 只,持续灌胃2 周,每天1 次。检测并记录体质量、疾病活动指数、结肠长度、结肠黏膜损伤指数、组织病理学改变和结肠组织学损伤指数,血清肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、IL-6,结肠水通道蛋白3(AQP3)、水通道蛋白4(AQP4)蛋白和mRNA。 结果 与空白组相比,模型组和给药组血清TNF-α、IL-1β、IL-6 浓度升高(P<0. 05);模型组和给药组结肠AQP3、AQP4 蛋白表达降低(P<0. 05);模型组、给药组结肠AQP3 mRNA 表达和模型组、西药组结肠AQP4 mRNA 表达降低(P<0. 05)。中药组结肠AQP4mRNA 表达与空白组相比无统计学差异(P>0. 05)。与模型组相比,给药组血清TNF-α、IL-1β、IL-6 浓度降低(P<0. 05);中药组血清TNF-α、IL-1β、IL-6 浓度比西药组更低(P<0. 05);给药组结肠AQP3、AQP4 蛋白和mRNA 表达升高(P<0. 05);中药组结肠AQP4 蛋白表达比西药组更高(P<0. 05);中药组结肠AQP3、AQP4 mRNA 表达比西药组更高(P<0. 05)。 结论 甘草泻心汤能有效改善UC 大鼠体质量、疾病活动指数、结肠黏膜损伤指数、组织学损伤指数等情况,其可能通过改善结肠AQP3、AQP4 蛋白和mRNA 表达,调节血清TNF-α、IL-1β、IL-6 水平,推测这些可能是甘草泻心汤治疗UC 的效应机制。
Objective To explore the effects of Gancao Xiexin decoction(GXD)on aquaporin(AQP)3 and AQP4 in rats with ulcerative colitis(UC). Methods Thirty-two clean SD rats, eight SD rats were used as the blank group, the remaining 24 rats were used to replicate the UC model with 4% dextran sulfate sodium. After the model was successful, the rats were divided into model, Western medicine(slow-release mesalazine granules), and Chinese medicine(Gancao Xiexin decoction)groups, eight rats in each group and then gavaged continuously for 2 weeks once a day. Body mass, disease +activity index, colon length, colonic mucosa damage index, histopathological changes, histological damage index, serum tumor necrosis factor-α (TNF-α), interleukin-(IL)-1β, IL-6 and colonic AQP3 and AQP4 were assessed. Results Compared with the blank group, serum levels of TNF-α, IL-1β, and IL-6 in model and administration groups were increased(P<0. 05), colonic AQP3 and AQP4 levels in model and administration groups were decreased(P<0. 05), and colonic AQP3 mRNA levels in model and administration groups, and AQP4 mRNA levels in model and Western medicine groups were declined(P< 0. 05). There was no remarkable difference in mRNA expression of colonic AQP4 between Chinese medicine and blank groups(P>0. 05). Compared with the model group, serum levels of TNF-α, IL-1β, and IL-6 in Western and Chinese medicine groups were decreased(P<0. 05), serum levels of TNF-α, IL-1β and IL-6 in the Chinese medicine group were lower than those in the Western medicine group(P<0. 05), colonic AQP3 and AQP4 protein and mRNA levels in administration groups were increased(P<0. 05), the colonic AQP4 protein level in the Chinese medicine group was higher than that in the Western medicine group(P<0. 05), and colonic AQP3 and AQP4 mRNA levels in the Chinese medicine group were higher than those in the Western medicine group(P<0. 05). Conclusions GXD effectively improves the body weight, disease activity index, colonic mucosa damage index, and histological damage index of UC rats, which may regulate serum TNF-α, IL-1β, and IL-6 levels by improving AQP3 and AQP4 protein and mRNA levels in the colon, which may be the mechanism of GXD in for UC treatment.
雷 娜,雷兰平,吴 容,权娜娜,唐学贵.甘草泻心汤对溃疡性结肠炎大鼠水通道蛋白3、4 的影响[J].中国比较医学杂志,2023,33(8):47~54.
复制