+高级检索
首页 > 过刊浏览>2023年第33卷第8期 >28-37. DOI:10. 3969 / j.issn.1671-7856. 2023. 08. 005
PDF HTML阅读 XML下载 导出引用 引用提醒
不同缺血时间对中国小型猪心肌缺血再灌注损伤的影响研究
作者:
作者单位:

1.中国中医科学院西苑医院中药药理北京市重点实验室,国家中医心血管疾病临床医学研究中心,北京 100091;2.黑龙江中医药大学,哈尔滨 150040

中图分类号:

R-33


Effects of various ischemia times on myocardial ischemia-reperfusion injury in the Chinese miniature swine model
Author:
  • YANG Lili 2,1

    YANG Lili

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • KANG Jing

    KANG Jing

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China. 2. Heilongjiang University of Chinese Medicine, Harbin 150040
    在期刊界中查找
    在百度中查找
    在本站中查找
  • REN Jianxun 2,1

    REN Jianxun

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • GUO Hao 2,1

    GUO Hao

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • MENG Hongxu 2,1

    MENG Hongxu

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • MA Yanlei 2,1

    MA Yanlei

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • SHI Yue 2,1

    SHI Yue

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LIU Jianxun 2,1

    LIU Jianxun

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Lei 2,1

    LI Lei

    1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China.
    在期刊界中查找
    在百度中查找
    在本站中查找
Affiliation:

1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China. 2. Heilongjiang University of Chinese Medicine, Harbin 150040

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献
    • | | | |
  • 文章评论
    摘要:

    目的 探讨不同缺血时间对中国小型猪急性心肌缺血再灌注损伤的影响,为建立适合的小型猪心肌缺血再灌注模型提供依据。 方法 中国小型猪29 只,随机分为假手术组(6 只)、缺血30 min 组(7 只)、缺血60 min 组(7 只)及缺血90 min 组(9 只)。各组戊巴比妥钠麻醉后行左冠状动脉造影,后将球囊经导丝交换入冠脉左前降支中部,术中连续心电监护记录ST 段改变情况、室颤及除颤次数,再灌注3 h 后处死。于缺血前、再灌即刻、再灌后1 h、2 h 及3 h 采集超声心动图,描记心脏收缩功能左心室射血分数(left ventricular ejection fraction, EF)及左心室短轴缩短率(left ventricular fractional shortening, FS);检测血清肌酸激酶同工酶(creatine kinase isoenzyme, CKMB)、乳酸脱氢酶(lactate dehydrogenase, LDH) 及超氧化物歧化酶(superoxide dismutase, SOD) 活性,丙二醛(malondialdehyde ,MDA)含量。TTC/ 伊文思蓝双重染色观察心肌梗死面积,硫磺素S 染色法观察再灌后无复流面积。 结果 缺血90 min 组动物死亡率较缺血30 min 组、缺血60 min 组高;随缺血时间增长,室颤及除颤次数明显增加(P<0. 05,P<0. 01);缺血90 min 组再灌注3 h 时EF 值、FS 值较其他组明显降低(P<0. 05,P<0. 01);模型组动物在心电图缺血性改变、心肌梗死及无复流面积、心肌组织染色及血清CK-MB、LDH、SOD、MDA 含量方面无显著性差异。 结论 心肌缺血90 min 再灌注小型猪模型的恶性心律失常及死亡率高,缺血30 min 再灌注3 h 条件建立的模型能复制临床疾病的特征性病变且死亡率低,适合于发病机制及药物作用研究。

    Abstract:

    Objective To investigate various ischemia times of myocardial ischemia-reperfusion injury (MI/ RI) in Chinese miniature swine and provide the basis to establish the optimal model of MI/ RI in Chinese miniature swine. Methods Twenty-nine Chinese miniature swine were randomly divided into a sham group (n=6), 30 min ischemia group (n=7), 60 min ischemia group (n=7), and 90 min ischemia group (n=9). After pentobarbital sodium anesthesia, left coronary angiography was performed in each model group, and a balloon was exchanged into the middle of the left anterior descending branch through a wire. We elevated the ST segment on a body surface electrocardiogram and the numbers of ventricular fibrillations and defibrillations during the operation. Left ventricular ejection fraction (EF) and left ventricular fractional shortening (FS) were recorded before the operation, before reperfusion, and at 1, 2 and 3 h of reperfusion. Serum levels of creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured at the same time. The area of myocardial infarction was observed by double staining with TTC/ Evans blue. The non-reflowed area after reperfusion was observed by thioflavin S staining. Results Mortality was the highest in the 90 min ischemia group. The numbers of ventricular fibrillations and defibrillations were increased significantly with increasing ischemia time (P<0. 05, P<0. 01). Ejection fraction and fractional shortening were obviously lower after 90 min of ischemia and ay 3 h of reperfusion compared with those in the other groups (P<0. 05, P<0. 01). No significant differences were found in the electrocardiogram, areas of myocardial infarction and non-reflow, myocardial tissue staining, or serum levels of CK-MB, LDH, SOD, and MDA among model groups. Conclusions A swine model of MI/ RI with 90 min of ischemia had strong arrhythmias and high mortality, whereas models established under ischemia for 30 min with reperfusion for 3 h replicated the characteristic lesions of clinical disease with low mortality and are suitable for studies of pathogenesis and drug actions.

    参考文献
    相似文献
    引证文献
引用本文

杨丽丽,康 静,任建勋,郭 浩,孟红旭,马彦雷,史 跃,刘建勋,李 磊.不同缺血时间对中国小型猪心肌缺血再灌注损伤的影响研究[J].中国比较医学杂志,2023,33(8):28~37.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-11-12
  • 在线发布日期: 2023-11-09
您是第 位访问者
版权所有:中国比较医学杂志 主管单位：中国科学技术协会 主办单位：中国实验动物学会 中国医学科学院医学实验动物研究所
地址：北京市朝阳区潘家园南里5号 邮 编 ：100021 电话：010-67779337 E-mail:bjb@cnilas.org
技术支持：北京勤云科技发展有限公司
防诈骗提示！请勿点击不明链接或添加个人微信。编辑部所有邮箱后缀均为@cnilas.org
关闭