Raising the microbial level of experimental mice by mouse rederivation has become essential in experimental animal research institutions, and it is an important technical method to guarantee the life quality of experimental mice. The current international mouse rederivation protocol is in vitro fertilization and embryo transplantation technology. The technology platform of mouse rederivation is comprehensive, covering various functional areas including a dirty mouse feeding room, mouse embryo operation room, surrogate mouse feeding room, and purified mouse feeding room. Considering the technology platform of mouse rederivation in the Animal Center of Zhejiang University as an example, the design strategies and operation mode were analyzed, and maintenance of the technology platform is discussed from the aspects of mouse microbial quality control, environmental sanitation control, and instrument and equipment maintenance to provide references for construction of the same type of technology platform.