虫草素通过调控miR-135b-5p 表达抑制宫颈癌Hela细胞侵袭、转移的实验研究
作者:
作者单位:

1.保定市妇幼保健院妇科,河北 保定 071000;2.定州市人民医院妇科,河北 定州 073000;3.河北省第七人民医院妇科,河北 定州 073000

中图分类号:

R-33


Cordycepin inhibits invasion and metastasis of Hela cervical cancer cells by regulating miR-135b-5p expression
Author:
Affiliation:

1. Department of Gynecology, Baoding Maternal and Child Health Hospital, Baoding 071000, China. 2. Department of Gynecology, Dingzhou People’s Hospital, Dingzhou 073000. 3. Department of Gynecology, Hebei Seventh People’s Hospital, Dingzhou 073000

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    目的 研究虫草素通过调控miR-135b-5p 表达对宫颈癌Hela 细胞侵袭、转移的影响及其调控机制。 方法 用浓度为50 μmol/ L(虫草素Ⅰ组)和100 μmol/ L(虫草素Ⅱ组)的虫草素处理宫颈癌Hela 细胞,另设空白对照组,用MTT 法测定Hela 细胞的生长情况。采用Transwell 法测定Hela 细胞侵袭情况,采用划痕实验检测宫颈癌Hela 细胞的转移能力,采用Real-time PCR 法测定人永生化表皮细胞Hacat 和宫颈癌Hela 细胞中miR-135-5p 的表达水平及不同浓度虫草素处理后对miR-135-5p 的表达水平的影响。转染miR-135b-5p mimic 至宫颈癌Hela 细胞,并采用Transwell 技术和划痕实验检测宫颈癌Hela 细胞的侵袭能力和转移能力。 结果 加入虫草素后宫颈癌Hela细胞生长受到显著的抑制,且高浓度的虫草素抑制作用更明显(P<0. 05)。宫颈癌Hela 细胞中miR-135b-5p 的表达水平为(1. 97±0. 07),显著高于人永生化表皮细胞Hacat 细胞中miR-135b-5p 的表达水平(1. 01±0. 03),组间比较差异具有统计学意义(t= 28. 187,P= 0. 000)。加入虫草素后,宫颈癌Hela 细胞侵袭率和转移率及miR-135b-5p表达明显降低;且高浓度虫草素处理后,宫颈癌Hela 细胞侵袭率和转移率更低,组间比较差异均具有统计学意义(t=138. 614~317. 100,P<0. 05)。过表达miR-135b-5p 显著增加宫颈癌Hela 细胞的侵袭率和转移率(t= 7. 145,t=7. 465,P<0. 05),且Vimentin 表达增加、E-cad 表达降低(t= 8. 223,t= 7. 473,P<0. 05)。 结论 虫草素可通过抑制miR-135b-5p 表达进而抑制Hela 细胞的侵袭和转移。

    Abstract:

    Objective To study the effect of cordycepin on invasion and metastasis of Hela cervical cancer cells by regulating miR-135b-5p expression and its regulatory mechanism. Methods Hela cells were treated with 50 and 100 μmol/ L cordycepin, and Hela cell growth was measured by MTT assays. Transwell assays were used to assess Hela cell invasion, a scratch assay was used to assess the metastasis ability of Hela cells, and Real-time PCR was used to measure the miR-135-5p expression level in human immortalized epidermal cell line Hacat and Hela cells, and the effect of various cordycepin concentrations on miR-135-5p expression level. miR-135b-5p mimic was transfected into Hela cells, and the invasion and metastasis of Hela cells were assessed by Transwell and scratch assays. Results After cordycepin treatment, Hela cell growth was significantly inhibited, and the inhibitory effect of the high concentration cordycepin was more obvious(P<0. 05). The miR-135b-5p expression level in Hela cells was (1. 97±0. 07), which was significantly higher than that in Hacat cells(1. 01±0. 03), and the difference between groups was statistically significant(t=28. 187, P=0. 000). After cordycepin treatment, the invasion and metastasis rates of Hela cells and miR-135b-5p expression were decreased significantly. After treatment with a high cordycepin concentration, the invasion and metastasis rates of Hela cells were lower, and the differences between groups were statistically significant(t= 138. 614~317. 100, P<0. 05). Overexpression of miR-135b-5p significantly increased the invasion and metastasis rates of Hela cells(t= 7. 145, 7. 465, P<0. 05), and vimentin expression was increased and E-cad expression was decreased(t= 8. 223, 7. 473, P< 0. 05). Conclusions Cordycepin inhibits the invasion and metastasis of Hela cells by inhibiting miR-135b-5p expression.

    参考文献
    相似文献
    引证文献
引用本文

魏杏茹,王 翠,庞旭娇,杜婷婷.虫草素通过调控miR-135b-5p 表达抑制宫颈癌Hela细胞侵袭、转移的实验研究[J].中国比较医学杂志,2023,33(6):68~74.

复制
分享
文章指标
  • 点击次数:488
  • 下载次数: 915
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2022-07-15
  • 在线发布日期: 2023-08-18
防诈骗提示!请勿点击不明链接或添加个人微信。编辑部所有邮箱后缀均为@cnilas.org
关闭