ABSL-3 实验室中恒河猴饲养的微生物风险控制
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中国医学科学院医学生物学研究所,昆明 650118

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R-33


Microbiological risk control for rhesus monkeys in animal biosafety level 3 laboratories
Author:
Affiliation:

Institute of Medical Biology, Chinese Academy of Medical Science, Kunming 650118, China

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    摘要:

    目的 在动物生物安全三级(ABSL-3)实验室内以指示微生物对恒河猴饲养及冲洗过程所产生的浮游菌气溶胶进行模拟检测。 方法 采用赛多利斯 Airport MD 8 便携式浮游菌采样仪对 ABSL-3 实验室猴笼地面排泄物及排水口滤篮处进行采样检测。检测方法包括:气溶胶活细胞检测及浮游菌 PCR 检测。 结果 (1)静止状态下有<5 个细菌生长;(2)饲养状态下,地面细菌培养计数达约 30 个/ 皿,酵母生长数为 1 个/ 皿,排水口的细菌计 数>300 个/ 皿,伴有>10 个/ 皿酵母生长;(3)对比试验结果显示,严格按照清洁处理 SOP 操作较未遵循 SOP 而言, 细菌及酵母生长明显减少,且在吸水垫上未检测到细菌 DNA。 结论 在 ABSL-3 实验室严格执行恒河猴饲养清洁 SOP 能够有效控制微生物污染的风险。

    Abstract:

    Objective To simulate the detection of plankton-aerosols generated by the rearing and flushing of rhesus monkeys using standard operating procedure ( SOP ) for microbes in an animal biosafety level 3 ( ABSL-3) laboratory. Methods The ground excreta and filter basket at the outlets of the monkey cages were measured during the stationary or feeding state. The SOP for feeding the monkeys and cleaning the cages was either not strictly followed or was strictly followed. A bacillus bacterium was used as the indicator microorganism and aerosols were collected using the Sedolis Airport MD 8 portable plankton sampler. The detection method included live cell cultures and PCR detection. Results (1) In the stationary state, there were < 5 bacteria. (2) In the feeding state, the bacterial culture count from the floor of the monkey cage was approximately 30 / dish. The yeast growth number was 1 / dish, and the bacterial count in the filter basket at the drain outlet was > 300 / dish accompanied by the growth of > 10 / dish. ( 3) The comparative test result demonstrated that the growth of bacteria and yeast was significantly reduced when strict SOP was followed for cleaning compared with not following the SOP. No bacterial DNA was detected on the absorbent mat. Conclusions The risk of microbiological contamination can be effectively controlled by strict implementation of SOPs for feeding and cleaning cages of rhesus monkeys in the ABSL-3 laboratory.

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梁 燕,刘龙丁,杨梦莉,李敬美,郭 磊,李 恒,郑惠文,李 菁,施海晶. ABSL-3 实验室中恒河猴饲养的微生物风险控制[J].中国比较医学杂志,2022,32(2):80~86.

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  • 收稿日期:2021-03-24
  • 在线发布日期: 2022-04-12
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