LncRNA SNHG1 对 IL-6 诱导的皮肤鳞状细胞癌细胞的增殖和迁移的影响
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江南大学附属医院皮肤科,江苏 无锡 214000

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R-33


Effect of the LncRNA SNHG1 on the proliferation and metastasis of skin squamous cell carcinoma induced by interleukin-6
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Affiliation:

Department of Dermatology, Affiliated Hospital of Jiangnan University, Wuxi 214000,China

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    摘要:

    目的 探讨长链非编码 RNA(LncRNA)SNHG1 对 IL-6 诱导的皮肤鳞状细胞癌细胞的增殖和迁移的影响。 方法 通过 qRT-PCR 检测 SNHG1 的相对表达;采用 CCK8 检测细胞活力;采用 Transwell 检测细胞的迁移和侵袭能力;采用 Western blot 检测 Ki67、MMP-2、E-cadherin、N-cadherin 和 Vimentin 蛋白表达。 结果 与正常皮肤细胞系 HaCaT,SNHG1 在皮肤鳞状细胞癌细胞系 A431、SCC13、SCL-1 中表达显著增加。 不同浓度的 IL-6 诱导 A431 细胞后,SNHG1 相对表达量、细胞活力、迁移细胞数目和侵袭细胞数目显著增加,及上调 Ki67、MMP-2、N-cadherin 和 Vimentin 蛋白表达,下调 E-cadherin 蛋白表达,呈剂量依赖性。 IL-6+si-SNHG1 组的 SNHG1 相对表达、细胞活力、迁移细胞数目、侵袭细胞数目、Ki-67、MMP-2、N-cadherin 和 Vimentin 蛋白表达显著低于 IL-6+si-NC 组,而 E-cadherin 蛋白表达显著高于 IL-6+si-SNHG1 组。 结论 干扰 SNHG1 表达可以抑制 IL-6 诱导的皮肤鳞状细胞癌细胞的增殖、 迁移、侵袭和上皮间质转化(EMT)。

    Abstract:

    Objective To investigate the effects of the long non-coding RNA ( LncRNA) SNHG1 on the proliferation and metastasis of skin squamous cell carcinoma induced by interleukin ( IL)-6. Methods The relative expression of SNHG1 was detected by qRT-PCR, and cell viability was detected using the cell counting kiT-8 assay. The migration and invasion ability of the cells was detected using the Transwell system, and protein expression of the proliferation marker Ki67, matrix metalloproteinase-2 (MMP-2), E-cadherin, N-cadherin and vimentin was detected by Western blot. Results Compared with that in the normal skin cell line HaCaT, the expression of SNHG1 was significantly increased in the skin squamous cell carcinoma lines A431, SCC13 and SCL-1. After A431 cells were treated with different concentrations of IL-6, the relative expression of SNHG1, cell viability, and number of migrating and invading cells increased significantly compared with that in untreated cells. Furthermore, the expression of Ki67, MMP-2, N-cadherin and vimentin protein was upregulated, whereas E-cadherin protein was downregulated after IL-6 treatment and the expression was dose-dependent. The relative expression of SNHG1, cell viability, number of migrating and invasive cells, and expression of Ki-67, MMP-2, N-cadherin, and vimentin proteins in the IL-6+si-SNHG1 group were significantly lower than those in the IL-6+si-NC group and the expression of E-cadherin protein was significantly higher than that in the IL-6+si- SNHG1 group. Conclusions Interference with SNHG1 expression inhibited the proliferation, migration, invasion and epithelial-mesenchymal transition of skin squamous cell carcinoma cells induced by IL-6.

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钟 咪,张佳音,饶美荣,曾 芬. LncRNA SNHG1 对 IL-6 诱导的皮肤鳞状细胞癌细胞的增殖和迁移的影响[J].中国比较医学杂志,2022,32(2):74~79.

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  • 收稿日期:2020-12-25
  • 在线发布日期: 2022-04-12
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