银杏蜜环口服溶液对大鼠心肌微血管内皮细胞血管新生能力的影响
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1.中国中医科学院西苑医院基础医学研究所,北京市中药药理重点实验室,北京 100091; 2.西安步长中医心脑病医院,西安 710082

中图分类号:

R-33


Effect of Yinxing Mihuan oral solution on angiogenesis of rat cardiac microvascular endothelial cells
Author:
Affiliation:

1.Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China. 2. Xi’an Buchang Traditional Chinese Cardio-cerebrovascular Disease Hospital, Xi’an 710082

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    摘要:

    目的 观察银杏蜜环口服溶液(简称银蜜)对 CMECs(大鼠心肌微血管内皮细胞)增殖、迁移、细胞小管形成及 VEGF(血管内皮生长因子)、CD31(血小板-内皮细胞粘附分子)蛋白表达的影响,探讨银蜜的体外血管新生能力。 方法 培养 CMECs,分为对照组、银蜜高( 2. 6 mg / mL)、中( 1. 3 mg / mL)、低( 0. 6 mg / mL) 剂量组。 CCK-8 法检测 CMECs 增殖能力;细胞划痕实验检测 CMECs 迁移能力;Matrigel 血管形成实验检测 CMECs 成管能力;Western blot 检测 VEGF、CD31 蛋白表达。 结果 与对照组相比,银蜜高、中、低剂量均能显著促进 CMECs 增殖; 且银蜜高组细胞增殖率显著高于银蜜中、低组。 划痕 6 h 后银蜜高组细胞迁移率显著高于对照组,银蜜中、低组细胞迁移率大于对照组,但无统计学差异;划痕 24 h 后银蜜高、中、低组 CMECs 迁移率均显著升高,银蜜高组细胞迁 移率高于银蜜中、低组。银蜜高组 CMECs 网眼数、网眼面积、交叉点数显著优于对照组、银蜜中、低组。 银蜜高、中组 VEGF、CD31 蛋白表达量显著升高。 结论 银杏蜜环口服溶液可促进 CMECs 的增殖、迁移和体外血管成环,同时促进 VEGF 和 CD31 蛋白表达,且药效随银蜜剂量的增加而增强,提示银杏蜜环口服溶液具有显著的促进体外血管新生能力。

    Abstract:

    Objective To determine the effects of Yinxing Mihuan (YM) oral solution on the proliferation, migration, tubule formation and protein expression of vascular endothelial growth factor (VEGF) and CD31 in rat cardiac microvascular endothelial cells (CMECs) and whether YM promotes angiogenesis in vitro. Methods CMECs were cultured and divided into a control group and YM high dose (2. 6 mg / mL), YM medium dose (1. 3 mg / mL) and low dose (0. 6 mg / mL) YM groups. The cell counting CCK-8 assay was used to measure proliferation, and the scratch test was used to detect the migration of CMECs. A Matrigel-based angiogenesis assay was used to detect tube formation by CMECs, and Western blot were used to detect the expression of VEGF and CD31. Results Compared with that in the control group, the high, medium and low YM doses significantly promoted the proliferation of CMECs, and the proliferation rate in YM high dose YM group was significantly higher than that in YM medium and low dose YM groups. Six hours after scratching the CMEC monolayer, the migration rate of CMECs in YM high dose YM group was significantly higher than that in the control group; the migration rates of CMECs in YM medium and low dose YM groups were increased, but there was no significant difference compared with the control group. Twenty-four hours after scratching the monolayer, the migration rates of CMECs in YM high, medium and low dose groups were significantly increased compared with that in the control group. The Matrigel matrix number, area and cross points for CMECs in YM high dose group were significantly improved compared with those in YM medium and low dose YM groups. Compared with that in the control group, the expression of VEGF and CD31 in YM high and medium dose YM groups was significantly increased. Conclusions YM can promote proliferation, migration, angiogenesis and expression of VEGF and CD31 proteins in CMECs in vitro. These effects were dose-dependent, suggesting that YM has a significant ability to promote angiogenesis.

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韩 笑,姚明江,任建勋,付建华,任钧国,金 龙,郭 浩,赵步长,王益民,刘建勋.银杏蜜环口服溶液对大鼠心肌微血管内皮细胞血管新生能力的影响[J].中国比较医学杂志,2022,32(2):22~28.

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  • 收稿日期:2021-03-26
  • 在线发布日期: 2022-04-12
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