Objective The quality of laboratory animals directly affects the quality of data they provide and has a substantial impact on public health and safety. Methods To improve the quality of laboratory animals, a management system and pathogen-detection scheme were implemented for the unique conditions in a university laboratory animal center with animals from different sources and high personnel traffic. Results The laboratory animal accreditation system, etiology surveillance system and animal-related supplies, was established and implemented. Since 2018, 1481 non-living animal samples were detected, 106 of which were pathogen-positive. In addition, 395 live animal samples were detected, 38 of which were pathogen-positive. A total of 363 animal-related supplies were monitored. Conclusions Several severe infectious-disease-agent-positive animals were forbidden to enter; opportunistic-agent-positive supply units were closely monitored; and the Objective monitoring of pathogen genealogy in rats and mice was adjusted and updated. Hence, the quality of the laboratory animals was evaluated in time to prevent false result .