北京协和医学院比较医学中心,中国医学科学院医学实验动物研究所,国家卫生健康委员会人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
R-33
ZHANG Jingjing
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaTONG Ling
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaLU Jiahan
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaLU Qiuhan
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaCONG Zhe
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaXUE Jing
Comparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, ChinaComparative Medicine Center, Peking Union College (PUMC)&Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS); NHC Key Laboratory of Human Disease Comparative Medicine; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine; Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
目的 通过建立 SIVmac239 潜伏感染恒河猴模型,研究 CD32 在静息型、活化型和/ 或记忆性 CD4+T 细胞亚群的表达特征,并分析其作为艾滋病潜伏库标志物的可能。 方法 SIVmac239 慢性感染的恒河猴给予联合抗逆转录病毒疗法,建立 SIVmac239 潜伏感染恒河猴模型。利用该模型,检测各阶段样本中血浆病毒载量、CD4+T 细胞计数、CD4 / CD8 细胞比值,及 CD32 在不同 CD4+T 细胞亚群中的表达变化。 结果 同感染前相比,CD32 在 SIV 感染后处于活化状态的幼稚 T 细胞中表达增加,在 HLA-DR+ CD4+T 细胞中表达增加,但 CD32 在静息型 CD4+T 细胞和各静息型记忆性 T 细胞亚群的表达无显著差异。 结论 本研究为 CD32 不是艾滋病潜伏库标记物这一观点提供数据支持,并为后续艾滋病治愈研究提供信息。
Objective We treated simian immunodeficiency virus ( SIV) mac239-infected rhesus macaque monkeys with antiretroviral therapy and examined the characteristics of CD32 expression on resting, activated, and / or memory CD4+ T-cell subsets, to determine their potential as a biomarker of HIV/ SIV reservoirs. Methods Four monkeys chronically infected with SIVmac239 were intramuscularly administrated antiretroviral therapy. Using this animal model, we detected plasma viral loads, CD4+T-cell counts, CD4 / CD8 ratios, and the percentage of CD32 expression in different CD4+T-cell subsets at different stages. Results Compared with the pre-infection stage, the percentage of CD32 expression in activated naive CD4+T cells and HLA-DR+ CD4+T-cell subsets was significantly increased at the post-infection stage. Moreover, there were no changes in the percentages of CD32 expression in resting CD4+T cells, resting naive CD4+T cells, resting central memory CD4+T cells, resting effector memory CD4+T cells, and resting TEMRA CD4+T cells. Conclusions This study provides more support for the view that CD32 is not the main biomarker of SIV reservoirs, and provides insight for future AIDS treatment.
张京京,童 玲,陆佳涵,卢秋翰,丛 喆,薛 婧. CD32 在艾滋病潜伏感染恒河猴模型的表达特征[J].中国比较医学杂志,2021,31(5):15~21.
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