跑台运动对脑卒中后抑郁大鼠抑郁样行为、海马氧化应激及海马 CA1 区 BDNF 表达的影响
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广东理工学院体育系, 广东 肇庆 526100

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R-33


Effects of treadmill exercise on depressive behavior, and hippocampal oxidative stress and CA1 BDNF expression, in post-stroke depression rats
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Affiliation:

Department of Physical Education Guangdong, Polytechnic College, Zhaoqing 526100, China

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    摘要:

    目的 观察脑卒中慢性应激大鼠抑郁样行为、海马氧化应激及海马 CA1 区脑源性神经营养因子 (BDNF)表达的变化和中等强度跑台运动的干预作用,探讨跑台运动影响卒中后抑郁(PSD)大鼠抑郁行为的可能机制。 方法 将 44 只大鼠随机分为 4 组:即假手术组(sham)、卒中组(MCAO)、卒中抑郁组(PSD)及 PSD 运动组 (PSD+E)。 采用大脑中动脉栓塞法制备大鼠缺血性卒中模型,随后慢性不可预见性应激(CUMS)4 周制备 PSD 大鼠模型,同时 PSD+E 组大鼠进行 4 周中等强度跑台运动。运动及 CUMS 结束后,蔗糖水偏好实验( SPT)及强迫游泳实验(FST)评估大鼠的行为学变化,行为学测试结束后试剂盒法检测海马组织中超氧化物歧化酶(SOD)及过氧化氢酶(CAT)活性和丙二醛(MDA)含量,免疫组化法测量大鼠海马 CA1 区 BDNF 的表达。 结果 与 sham 组比较, PSD 大鼠 FST 中大鼠不动时间延长,SPT 中糖水摄入量及糖水偏爱百分比均显著减少;海马 SOD 及 CAT 活性显著下降,MDA 含量增加,海马 CA1 区 BDNF 数目显著减少。跑台运动干预后与 PSD 组大鼠比较,PSD+E 组大鼠不动时间缩短,糖水摄入量及糖水偏爱百分比均显著增多;大鼠海马 SOD 及 CAT 活性增强,MDA 含量减少,海马 CA1 区 BDNF 个数显著增多。 结论 跑台运动可以改善 PSD 大鼠抑郁样行为,可能与此运动增强 PSD 大鼠海马组织抗氧化能力及海马 CA1 区 BDNF 表达,从而对 PSD 大鼠起到保护作用有关。

    Abstract:

    Objective To observe changes in depressive-like behavior, and expression of oxidative stress markers and brain-derived neurotrophic factor (BDNF) in the CA1 hippocampus in post-stroke depression (PSD) model rats, and to investigate intervention with treadmill exercise in these rats and its possible mechanisms. Methods Forty-four male Sprague-Dawley rats were randomly divided into the following four groups: sham-operation, middle cerebral artery occlusion (MCAO), PSD, and PSD rats treated with 4 weeks of treadmill exercise only (PSD+E). Complete cerebral ischemic rat models of PSD were established by MCAO followed by 4 weeks of chronic unpredictable mild stimulation. Changes in rat behavior were evaluated by forced swimming test (FST) and sugar water preference test (SPT). The activities of superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) in the hippocampus, were detected using kits. Immunohistochemical staining was used to detect BDNF expression in the CA1 hippocampus. Results Compared with the sham group, there was prolongation of the FST immobility time, while sucrose water intake and sucrose consumption ratio in the SPT were significantly decreased in the group. In the PSD group, there was reduced SOD and CAT activity, and increased MDA concentration, in the hippocampus, while the level of BDNF in the hippocampal CA1 of rats was significantly reduced compared with the group. Compared with the PSD group, the PSD + E rats had shorter FST immobility times, significantly higher sucrose water intake and sucrose consumption ratios in the SPT, enhanced SOD and CAT activity, decreased MDA concentration in the hippocampus, and significantly increased expression of BDNF in the hippocampal CA1. Conclusions Treadmill exercise alleviated depressive-like behavior and conferred neuroprotection by reinforcing antioxidant capability in the hippocampus and activating BDNF expression in the hippocampal CA1 of PSD model rats.

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焦健华,高珊珊.跑台运动对脑卒中后抑郁大鼠抑郁样行为、海马氧化应激及海马 CA1 区 BDNF 表达的影响[J].中国比较医学杂志,2021,31(3):61~66.

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  • 收稿日期:2020-07-23
  • 在线发布日期: 2021-04-30
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