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首页 > 过刊浏览>2020年第30卷第12期 >57-63. DOI:10. 3969 / j.issn.1671-7856. 2020. 12. 009
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SD 大鼠老龄化过性程别中外周血究及免疫细胞表型的性别差异研究
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中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重 点实验室,北京市人类重大疾病实验动物模型工程技术研究中心,北京 100021

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R-33


Gender differences in peripheral blood and immune cell phenotypes in aging Sprague-Dawley rats
Author:
  • GUAN Bowen

    GUAN Bowen

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100021, China
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  • LU Yanhua

    LU Yanhua

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100022, China
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  • BAI Lin

    BAI Lin

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100023, China
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  • SHI Guiying

    SHI Guiying

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100024, China
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  • SU Lulu

    SU Lulu

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100025, China
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  • WANG Yuquan

    WANG Yuquan

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100026, China
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  • WEI Qiang

    WEI Qiang

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100027, China
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  • WANG Wei

    WANG Wei

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100028, China
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  • MENG Aimin

    MENG Aimin

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100029, China
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Affiliation:

Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; NHC Key Laboratory of Human Disease Comparative Medicine; Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100021, China

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    摘要:

    目的 比较分析不同月龄 SD 大鼠外周血和免疫器官的免疫细胞分型指标性别差异。 方法 选用 12 月龄 SD 大鼠和 2 月龄 SD 大鼠,雌雄各半,检测外周血细胞计数及分类、脏器指数、外周血和脾免疫细胞分型、 脾 T 细胞 P16 表达量,并对结果进行性别差异比较。 结果 年轻大鼠的各项检测指标未见性别差异。 外周血计数与分类结果显示,与青年大鼠比较,老年雌性大鼠白细胞下降;老年雄性大鼠和老年雌性大鼠红细胞上升,两项指标均具有性别差异;老年雄性大鼠和老年雌性大鼠均表现为中性粒百分比上升、淋巴细胞百分比下降、嗜酸性粒细胞百分比升高,未见性别差异;外周血其他指标未见明显改变。 老年雌性大鼠胸腺指数低于老年雄性大鼠;老年雄性大鼠脾指数升高,并高于老年雌性大鼠。 外周血免疫细胞流式分析结果显示,老年雌性大鼠辅助 T 细胞、调节 T 细胞和细胞毒性 T 细胞上升;老年雌性大鼠 B 细胞下降;老年雄性大鼠自然杀伤细胞升高;这几项指标均具有性别差异。 脾淋巴细胞分型结果显示,老年雌性大鼠 CD3+升高,CD45RA+下降。 脾 T 细胞 P16 表达量老年大鼠 P16 均升高,未见性别差异。 结论 本研究各项指标在青年组大鼠未见性别差异,老年大鼠在外周血白细胞、红细胞计数,中性粒、单核细胞百分比;免疫脏器指数;免疫细胞分型分析中调节 T 细胞,细胞毒 T 细胞,自然杀伤细胞,B 细 胞,CD3 阳性细胞等指标改变均存在性别差异。 提示老年大鼠的免疫系统的内稳态(homeostasis)平衡失调,在老年性改变中的一些指标出现性别差异。 本研究结果为研究老年疾病及衰老动物模型提供了性别对老龄化影响的基础数据。

    Abstract:

    Objective Gender differences in immune cell types in peripheral blood and spleen were compared between aged and young Sprague-Dawley rats. Methods Twelve-month and 2-month-old rats were used. Rats in each age group were half male and half female. Classification and counts of peripheral blood cells, organ index, peripheral blood and spleen immune cell typing, and P16 expression in spleen T cells were determined and the result compared with respect to age and gender differences. Results The peripheral blood leucocyte count was decreased in the aged female rats, and there was a gender difference. The erythrocyte count was increased in both aged male rats and aged female rats, but there was a difference between them. The neutrophil percentage was increased and the lymphocyte percentage was decreased in the aged male and female rats. The eosinophil percentage was increased in the aged male and female rats. The monocyte percentage in the aged female rats was lower than that in the aged male rats. No significant changes in other peripheral blood indices were found. The thymus index of the aged female rats was decreased and was lower than that of the aged male rats. The spleen index of the aged male rats was increased and was higher than that of the aged female rats. Peripheral blood immunophenotyping indicated that the percentages of T helper cells, regulatory T cells and cytotoxic T cells were increased in the aged female rats. The percentage of B cells was decreased in the aged female rats. The percentage of natural killer cells was increased in the aged male rats. Splenic lymphocyte typing showed that numbers of CD3+ cells increased and CD45RA+ cells decreased in aged female rats. P16 expression in splenic T cells increased in all aged rats, and no gender difference was observed. Conclusions No gender differences were found in the various indices among young rats. Some indices showed a gender difference in aged rats, including peripheral blood leucocyte count, erythrocyte count, neutrophil percentage, monocyte percentage, thymus index, spleen index, and immune cell typing for regulatory T cells, cytotoxic T cells, natural killer cells, B cells and CD3-positive cells. These result indicate altered immune system homeostasis in aged rats, leading to gender differences in some indices in aged rats. The present result provided basic data for studying the impact of gender on aging-related diseases and animal models of aging.

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管博文,卢延华,白 琳,石桂英,苏路路,王玉全,魏 强,王 卫,孟爱民. SD 大鼠老龄化过性程别中外周血究及免疫细胞表型的性别差异研究[J].中国比较医学杂志,2020,30(12):57~63.

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  • 收稿日期:2020-05-03
  • 在线发布日期: 2021-02-10
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