The Animal biosafety level 3 laboratory is a basic support platform of the national biosecurity system for responding to emerging and re-emerging communicable disease. It is also an important hardware foundation for studying the biological characteristics, prevention, and therapeutic tools of the novel 2019 coronavirus. In the emergency operation associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the development of knowledge of the virus, systematic and standardized biosafety management measures are essential for the efficient and orderly operation of the Biosafety Laboratory, and are critical in preventing the occurrence of accidents in the laboratory. This article discusses the biosafety management of the urgent development of SARS-CoV-2 experimental activities in the Animal biosafety bevel 3 baboratory, and aims to provide ideas for emergency management of related laboratories.