Myocardial infarction (MI) is a disease that causes an interruption in coronary blood flow. Results ing in ischemic myocardial necrosis. The clinical symptoms mainly include myocardial pain, heart failure, arrhythmia, and shock. The incidence of global myocardial infarction is increasing year by year, and patients are becoming younger. Clinical diagnosis and treatment method are largely improved, and the effect is remarkable. however, because of the universality and adaptability of the disease, its occurrence and development remain the focus of cardiovascular disease research. With the development of science and technology, molecular imaging has become more cutting-edge and diverse, and multiple technologies including animal ultrasound, CT, MRI, SPECT/ CT, and PET/ CT have achieved a series of outcomes in the diagnosis and treatment of myocardial infarction. This article summarizes the current application of molecular imaging technology to animal models of myocardial infarction, details its developmental trends and significance, and provides important references for clinical diagnosis and treatment.