中国农业科学院兰州兽医研究所 家畜疫病病原生物学重点实验室 农业部兽医公共卫生重点实验室, 兰州 730046; 2.西北民族大学生命科学与工程学院,兰州 730030
WANG Cong
1.State Key Laboratory of Veterinary Etiological Biology / Key Laboratory of Veterninary Public Health of Ministry of Agriculture / Lanzhou Veterinary Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. 2. College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030JING Zhizhong
1.State Key Laboratory of Veterinary Etiological Biology / Key Laboratory of Veterninary Public Health of Ministry of Agriculture / Lanzhou Veterinary Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. 2. College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 7300301.State Key Laboratory of Veterinary Etiological Biology / Key Laboratory of Veterninary Public Health of Ministry of Agriculture / Lanzhou Veterinary Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. 2. College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030
探讨抗性/ 易感小鼠 DNA 识别受体的组织表达差异与抗病性的关系。 方法 不同抗性品系小鼠感 染 ECTV 后,采用实时荧光定量 PCR(qRT-PCR)技术分析其组织的重要 DNA 识别受体差异表达谱。 结果 TLR9、 DAI、cGAS、RNA pol III 和 RIG-I 这 5 个与 DNA 识别相关的受体在不同抗性小鼠组织中均呈广泛性表达,但鼠痘抗 性小鼠 C57BL/ 6 品系的表达明显整体高于 BALB/ c 小鼠;在 ECTV 感染后易感小鼠 BALB/ c 品系的这些基因表达 明显上调,而 C57BL/ 6 小鼠变化不大甚至下调。 结论 这种差异表达特征表明这些受体分子可能与小鼠的天然抗 病力密切相关。
Objective To explore the relationship between disease resistance and susceptibility inmice expressing different levels of DNA pattern recognition receptors(PRRs). Methods The expression profiles of different DNA PPRs in BALB/ c and C57BL/ 6 mice infected with ECTV were detected using real-time fluorescent quantitative PCR ( qRT-PCR). Results TLR9, DAI, cGAS, RNA pol Ⅲ and RIG-Ⅰ were expressed in both mouse lines, but were expressed at significantly higher levels in the mousepox-resistant C57BL/ 6 mice compared with BALB/ c mice prior to infection. However, the expression of these genes increased noticeablyin the ECTV-susceptible BALB/ c mice after infection, with little change seen in C57BL/ 6 mice after infection. Conclusions Our findings suggest that these pathogen-specific PPRsmay be closely related to natural disease resistance in mice.
何小兵,王 聪,成温玉,贾怀杰,陈国华,房永祥,刘翊中,景志忠.不同抗性小鼠 ECTV 感染前后重要模式识别受体的组织表达差异分析[J].中国比较医学杂志,2020,30(7):13~22,109.
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