1.中国食品药品检定研究院 实验动物资源研究所,北京 102629; 2.中国食品药品检定研究院 食品化妆品检定所, 北京 100050; 3.中国农业大学动物医学院,北京 100193
R-33
1.Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 102629, China. 2. Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050. 3. College of Veterinary Medicine, China Agricultural University, Beijing 100193
目的 评价基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)检测实验动物病原菌的效果。 方法 使用德国 Bruker Biotyper 系统对 40 株标准菌株及 384 株实验动物分离菌进行鉴定。 结果 Bruker Biotyper 检测标准菌株和分离株的准确率分别为 90. 0%和 80. 0%。 对鼠伤寒沙门氏菌( Salmonella typhimurium)、假结核耶尔森氏菌(Yersinia pseudotuberculosis)、志贺菌( Shigella spp.)、肺炎克雷伯杆菌(Klebsiella pneumoniae)、嗜肺巴斯德杆菌 Heyl 生物型 ( Rodentibacter heylii) 和 支 气 管 鲍 特 杆 菌 ( Bordetella bronchiseptica) 的 鉴 定 存 在 偏 差。 结论 MALDI-TOF MS 方法高效、准确率高,可用于实验动物病原菌的快速鉴定
Objective To evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detection of pathogenic bacteria in laboratory animals. Methods In total, 40 standard strains and 384 laboratory animal isolates were identified using the Bruker Biotyper system. Results The accuracy rates of Bruker Biotyper to detect standard strains and isolates were 90. 0% and 80. 0%, respectively. There were biases in the identification of Salmonella typhimurium, Yersinia pseudotuberculosis, Shigella spp., Klebsiella pneumoniae, Pasteurella pneumotropica biotype Heyl, and Bordetella bronchiseptica. Conclusions The MALDI-TOF MS method is highly efficient and accurate. It can be used for rapid identification of pathogenic bacteria in laboratory animals
邢 进,刘 娜,冯育芳,张雪青,崔生辉,贺争鸣,赵德明,岳秉飞.基质辅助激光解吸电离飞行时间质谱检测实验动物病原菌效果初探[J].中国比较医学杂志,2020,30(3):83~88.
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