Objective To examine blood physiological and serum biochemical values of Tim4 knock-in C57BL/ 6 mice ( hTIM4-EGFP). Methods Collected blood samples were tested for 22 physiological values and 12 biochemical values using an auto-analyzer. Data were analyzed by appropriate statistical method . Results Ten physiological and four biochemical values were significantly different between transgenic and wild-type groups (WBC, RBC, HGB, HCT, MCH, RDW, PLT, NEUT, LYM%, NEUT%, ALT, AST, TG, and P). Nine values were different between males and females in the transgenic group ( MCV, MCH, MCHC, RDW, PLT, NEUT, NEUT%, ALP, CHO), while ten values were different between sexes in the wild-type group (RBC, HCT, MCV, LYM, NEUT, LYM%, NEUT%, AST, ALT, and P). Conclusions Differences in blood physiological and serum biochemical values were observed between Tim4 knock-in and C57BL/ 6 mice. This study provides support for the application of hTIM4-EGFP.