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首页 > 过刊浏览>2020年第30卷第1期 >57-67. DOI:10. 3969 / j.issn.1671-7856. 2020. 01. 010
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小鼠侧脑室下区基因表达的增龄性变化研究
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中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京 100021

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R-33


Age-related changes in gene expression of the subventricular zone of mice
Author:
  • HUANG Yiying

    HUANG Yiying

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • SHI Guiying

    SHI Guiying

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • LYU Ying

    LYU Ying

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • LI Keya

    LI Keya

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China
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  • BAI Lin

    BAI Lin

    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China
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Affiliation:

Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine, Beijing 100021, China

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    摘要:

    目的 研究幼年期、成年期和老年期小鼠侧脑室下区( subventricular zone,SVZ)中基因表达的增龄性变化。 方法 分别提取3周龄、6月龄和 20月龄C57小鼠SVZ的mRNA,基于转录组测序及实时荧光定量 PCR 技术,检测 SVZ 中在不同年龄阶段的基因表达差异。 结果 以幼年期小鼠为对照,成年期检测到253个差异表达的基因,老年期检测到 519个,以成年期作对照,老年期检测到147个差异表达的基因。GO 分析发现成年期小鼠 与幼年期相比差异表达基因主要涉及营养和代谢,老年期小鼠与成年期相比主要涉及中枢神经系统神经元分化等。KEGG 分析发现差异表达基因主要涉及通路为蛋白质消化吸收、神经活性配体-受体相互作用等。 结论 该研究提供了不同年龄段SVZ 转录水平基因变化的数据,为探讨SVZ 的功能发育和退化,进一步治疗和抵抗神经衰 老提供新的理论依据和方法。

    Abstract:

    Objective To study the age-related changes in SVZ gene expression of 3-week-old, 6-month-old, and 20-month-old mice. Methods RNA was extracted from the SVZ of mice at the three ages. The difference in gene expression of SVZ at the different ages was detected by RNA sequencing and quantitative real-time PCR. Results Compared with the levels in young mice, 253 differentially expressed genes were detected in adult mice and 519 in old mice. Compared with the levels in adult mice, 147 differentially expressed genes were detected in old mice. GO analysis found that the differentially expressed genes are mainly involved in nutrition and metabolism in adult mice compared with young, and central nervous system neuron differentiation among others in old mice compared with adult mice. KEGG analysis found that the differentially expressed genes are mainly involved in protein digestion and absorption, and neuroactive ligand–receptor interaction, among others. The differentially expressed genes were analyzed by GO and KEGG pathways analyses. Conclusions This study provides basic data on the changes of gene transcription levels with age in the SVZ, and also provides a new theoretical basis for treatment and resistance to nerve aging in the future.

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黄艺滢,石桂英,吕颖,李珂雅,白琳.小鼠侧脑室下区基因表达的增龄性变化研究[J].中国比较医学杂志,2020,30(1):57~67.

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历史
  • 收稿日期:2019-08-23
  • 在线发布日期: 2020-08-19
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