Objective To analyze and evaluate the gait changes of Parkinson’s disease in mice using the CatWalk gait analysis system. Methods A Parkinson’s disease mouse model was established by the intraperitoneal injection of MPTP. After the model had been successfully established, the CatWalk gait analyzer was used to measure the relevant gait parameters, and the result were statistically analyzed. Results Compared with the control group, the pace of the mice in the model group was disordered. The run duration, maximum variation, stance, and step cycle were increased significantly in the MPTP group. However, the average speed, cadence, swing speed, and stride length were significantly reduced in the model group. Conclusions CatWalk can be used as an intuitive, precise, and effective new method to evaluate Parkinson’s mouse models.