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首页 > 过刊浏览>2019年第29卷第1期 >80-83. DOI:10.3969/j. issn. 1671 -7856. 2019. 01. 014
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小鼠局部淋巴结实验(BrdU-ELISA)在25%氰氟草酯乳油皮肤致敏性检测中的应用
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(1. 江苏省医药农药兽药安全性评价与研究中心,南京 211166;2. 南京医科大学公共卫生学院,南京 211166)

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R-33


Application of local lymph node assay (BrdU-ELISA) for the detection of 25% cyhalofop-butyl EC skin sensitization in mice
Author:
Affiliation:

(1. The Safety Assessment and Research Center for Drugs of Jiangsu Province, Nanjing 211166, China.2. School of Public Health, Nanjing Medical University, Nanjing 211166)

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    摘要:

    目的 采用小鼠局部淋巴结实验(BrdU-ELISA)法检测25%氰氟草酯乳油的致敏性。方法 1. 选用雌性BALB/ C 小鼠进行实验,受试样品设为100%、50%、25%,同时设阴性对照组[丙酮:橄榄油(acetone: olive oil,AOO)= 4∶1]、阴性对照组(橄榄油)及阳性对照组(25%已基肉桂醛),连续双耳背面染毒3 d,第6 天腹腔注射BrdU 溶液(5 mg/ 只),24 h 后采集耳后淋巴结称重等并通过BrdU-ELISA 实验方法检测淋巴增殖情况。结果 各剂量组的小鼠体重与对照组比较,差异均无显著性( P > 0. 05);各剂量组的耳厚度增加和耳重均不超过25%;溶剂和空白对照组的刺激指数SI1和SI2 <1. 6,阳性对照组(25%已基肉桂醛)的刺激指数SI1和SI2 >1. 6,实验系统可靠;25%氰氟草酯乳油低、中、高剂量组的刺激指数SI1和SI2 <1. 6,无剂量反应关系。结论 25%氰氟草酯乳油的小鼠局部淋巴结实验结果为阴性,即25%氰氟草酯乳油为无致敏性。

    Abstract:

    Objective To detect 25% cyhalofop-butyl emulsifiable concentrate(EC) skin sensitization by a local lymph node assay (BrdU-ELISA) in mice. Methods Female BALB/ C mice were tested and the samples were set up to 100%, 50%, and 25%. The negative control group received acetone: olive oil (AOO)= 4 ∶1, and the positive control group received 25% basal cinnamaldehyde. Test samples were laid on the dorsum of bilateral mouse ears for three consecutive days, then the mice received an intraperitoneal injection of BrdU (5 mg/ mouse) on the sixth day. After 24 hours, the lymph nodes were removed, weighed, and mashed into a cell suspension. Lymphocyte proliferation was measured by BrdU-ELISA. Results The body weights of female mice in all treated groups were similar to the control group. The increase of ear thickness and ear weight in each dose group was not more than 25%. The stimulus indexes of solvent and blank control groups were SI1and SI2< 1. 6, and the stimulation indexes of the positive control group (25% hexyl cinnamaldehyde) were SI1and SI2 > 1. 6. This indicated the test system was reliable. The stimulation indexes of 25%cyhalofop-butyl EC in the low, medium and high dose groups were SI1and SI2 < 1. 6, and there was no dose-response relationship. Conclusions The results of 25% cyhalofop-butyl EC in mouse local lymph node assays were negative,indicating that 25% cyhalofop-butyl does not cause skin sensitization.

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秦珩,程洁,靳苏香,钱雯,章婉,严婷,董昊,张成香,张璐璐,王玉邦,环飞.小鼠局部淋巴结实验(BrdU-ELISA)在25%氰氟草酯乳油皮肤致敏性检测中的应用[J].中国比较医学杂志,2019,29(1):80~83.

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  • 收稿日期:2018-07-17
  • 在线发布日期: 2019-02-11
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