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首页 > 过刊浏览>2019年第29卷第1期 >35-40. DOI:10.3969/j. issn. 1671 -7856. 2019. 01. 006
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阿司匹林对控制大鼠盐敏感性高血压的作用机制研究
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(1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,北京 100021;2. 北京市人类重大疾病实验动物模型工程技术研究中心,北京 100021;3. 首都医科大学,北京 100069)

中图分类号:

R-33


The mechanism of aspirin treatment for salt-sensitive hypertension in rats
Author:
  • LIU Xue

    LIU Xue

    1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021.
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  • YANG Yuhui 3

    YANG Yuhui

    Capital Medical University, Beijing 100069
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  • FU Hui

    FU Hui

    1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021.
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  • LIU Xing

    LIU Xing

    1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021.
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  • JIANG Xiaoliang

    JIANG Xiaoliang

    1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021.
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  • YANG Zhiwei

    YANG Zhiwei

    1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021.
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Affiliation:

(1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center,Peking Union Medical College (PUMC); Key Laboratory of Human Disease Comparative Medicine, National Health Commission,Beijing 100021, China. 2. Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021. 3. Capital Medical University, Beijing 100069)

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    摘要:

    目的 探讨阿司匹林对大鼠盐敏感性高血压的调节作用及相关机制。方法 2 月龄盐敏感性大鼠(Dahl SS) 及其对照盐耐受性大鼠(SS-13BN)分别给予低盐(0. 12% NaCl,LS)、高盐(8% NaCl,HS)、高盐加阿司匹林灌胃((10 mg/ (kg·d)),HS+ASA)饲养至8 周,期间尾袖法连续测量动脉血压,8 周后颈总动脉插管测量大鼠动脉血压,Real-time PCR 检测肾组织炎症因子IL-6、IL-1β、TNF-α 的表达,免疫荧光检测皮肤M2 型巨噬细胞的数量,免疫印迹检测血管功能代表性蛋白eNOS、vWF 的表达。结果 Dahl SS 大鼠高盐喂养后,血压明显升高,肾组织炎症因子和血管vWF 因子表达量显著增加、皮肤M2 型巨噬细胞数量及血管eNOS 表达量明显减少,阿司匹林能够有效改善Dahl SS 大鼠高盐诱导的血压升高、炎症反应及血管功能的损伤,而SS-13BN 大鼠并未出现上述情况。结论 阿司匹林通过抗血小板功能抑制了Dahl SS 大鼠由高盐诱导的炎症反应,抑制了血管功能损伤及高血压发生发展。

    Abstract:

    Objective To investigate the mechanism of aspirin on high salt-induced hypertension in Dahl saltsensitive rats. Methods Two-month-old Dahl salt-sensitive rats (Dahl SS) and salt-resistant rats (SS-13BN) were fed with Low salt (0. 12% NaCl, LS), High salt (8% NaCl, HS), High salt + Aspirin (8% NaCl +10 mg/ (kg·d)aspirin),or HS+ASA for 8 weeks. Blood pressure was measured by the tail cuff method. The expressions of renal inflammatory cytokines (IL-6, IL-1β, TNF-α) and eNOS and vWF in arteries were measured by real-time PCR and western blot,respectively. The number of skin M2 macrophages was measured by immunofluorescence. Results Blood pressure,expressions of renal IL-6, IL-1β, TNF-α, and arterial vWF were markedly increased, and the number of M2 macrophages in the skin, and eNOS expression in the arteries were significantly decreased in the high salt group compared with the low salt group. Aspirin inhibited all these events in rats on a high salt diet. However, the influence of high salt and aspirin was not observed in SS-13BN rats. Conclusions Aspirin attenuated salt-sensitive hypertension and arterial injury induced by a high salt diet in Dahl salt-sensitive rats via inhibiting platelet activated inflammation.

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刘雪,杨宇辉,付慧,刘星,姜晓亮,杨志伟.阿司匹林对控制大鼠盐敏感性高血压的作用机制研究[J].中国比较医学杂志,2019,29(1):35~40.

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  • 收稿日期:2018-09-11
  • 在线发布日期: 2019-02-11
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