(1. 河北医科大学第四医院消化内科,石家庄 050011; 2. 河北省优抚医院内科,石家庄 050051)
R-33
河北省自然基金课题资助项目(H2015206461)
(1. Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China; 2. The Special Care Hospital of Hebei Province, Shijiazhuang 050051)
目的 利用建立的肾性贫血大鼠模型,评价左卡尼汀联合促红细胞生成素(EPO)和铁剂对大鼠肾性贫血的治疗效果?方法 采用腺嘌呤灌胃建立肾性贫血大鼠模型,随机分为三组,对照组(A 组)?EPO 联合铁剂治疗组(B 组)?左卡尼汀联合EPO 和铁剂治疗组(C 组),连续给药3 周?分别进行外周血的RBC?Hgb?Hct?CRP?血清铁(Fe)?血清铁蛋白(SF)?转铁蛋白饱和度(TS)检测和肝?肾?小肠组织病理学分析?结果 B 组和C 组的RBC?Hgb?Hct?Fe?SF?TS 的指标水平较A 组有不同程度的上升,而CRP 下降明显,差异有显著性( P < 0.05);两个药物治疗组间,C 组RBC?Hgb?CRP 水平均高于B 组,差异有显著性( P < 0.05);组织病理学分析发现两个药物治疗组的肝细胞弥漫性水肿及点状坏死症状减少;肠黏膜溃疡减轻?萎缩面积缩小?结论 应用左卡尼汀联合EPO和铁剂治疗可显著改善大鼠肾性贫血,值得进一步研究推广?
Objective To investigate the therapeutic effect of L?carnitine combined with erythropoietin (EPO) and iron on renal anemia in rats. Methods The renal anemia rat model was established using adenine. All rats were randomly divided into three groups: control group (group A), EPO combined with iron group (group B), and L?carnitine combined with EPO and iron treatment group (group C). The levels of red blood cells (RBC), hemoglobin (Hgb), hematocrit (Hct), C?reactive protein (CRP), Fe in peripheral blood (Fe), serum ferritin (SF) and transferrin saturation (TS) were measured. Pathological changes in the kidney, liver and small intestine tissues were examined using HE staining. Results Compared with the group A, the levels of RBC, Hgb, Hct, Fe, SF, and TS were significantly increased, however, the level of CRP significantly decreased in the groups B and C (P < 0.05). In the group C, the levels of RBC, Hgb, and CRP was higher than the group B (P < 0.05). Furthermore, the pathological changes in the kidney, liver and small intestine tissues were improved in the groups B and C. Conclusions L?carnitine combined with EPO and iron has a therapeutic effect on renal anemia in rats, thus, it deserves further studies and application.
张风宾,赵飞,王英南,张瑞星.左卡尼汀联合EPO 和铁剂治疗大鼠肾性贫血[J].中国比较医学杂志,2018,28(6):29~33.
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