Objective To explore the population reproductive regularity and reproductive performance of rhesus and cynomolgus monkeys in Kunming area, and to provide reference data for the construction of rhesus and cynomolgus monkey breeding base, reproductive biology research and biological resources protection. Methods The reproductive regularity and reproductive performance in a rhesus monkey population of 20 males and 130 females, and a cynomolgus monkey population of 120 males and 780 females in a large-scale experimental monkey breeding base in Kunming were observed for a whole year and statistically analyzed. Results There was a distinct seasonal variation in the reproduction of rhesus monkeys in Kunming area, while no obvious seasonal changes was observed in cynomolgus monkeys. The pregnancy rate, reproductive rate and neonatal survival rate of rhesus monkeys were 76.15%, 69.23% and 90.70%, respectively. The pregnancy rate, neonatal reproduction rate and survival rate of cynomolgus monkeys were 78.98%, 74.87% and 94.81%, respectively. The menstrual cycle and pregnancy period of rhesus monkeys were (28.80 ±2.33) d and (165.87 ±7.52) d, respectively. The menstrual cycle and pregnancy period of cynomolgus monkeys were (29.35 ±3.05) d and (157.93 ±5.42) d, respectively. The average birth weight and weaning weight of rhesus monkey pups were (425.00 ±100.50) g and (1491.67 ±172.35) g, and those of cynomolgus monkeys were (314.33 ±61.18) g and (1013.50 ±115.50) g, respectively. Conclusions The reproduction regularity of rhesus and cynomolgus monkeys in Kunming area are defined. The reproductive performance of rhesus and cynomolgus monkeys are reported in detail. It provides basic data for the breeding of rhesus and cynomolgus monkeys in Kunming area and the development of research of experimental monkey reproductive biology.