Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China 在期刊界中查找 在百度中查找 在本站中查找
Objective To investigate the effect of Smad3 on cell migration of A549 and HeLa cells. Methods Primers for pCMV-Myc-Smad3 plasmid construction and siRNA targeting Smad3 were designed and synthesized. pCMV-Myc-Smad3 plasmid was constructed with molecular cloning techniques. Overexpression of Smad3 with Myc-tag or silencing of endogenous Smad3, and then scratch assay was used to detect the migration ability of A549 and HeLa cells in vitro. Results pCMV-Myc-Smad3 plasmid was successfully constructed. Overexpression of Smad3 significantly up-regulated the migration rate of A549 and HeLa cells. Conversely, in the same cells, silencing of endogenous Smad3 or treatment with Smad3 inhibitor, SIS3, down-regulated the migration rate. Conclusions Smad3 promotes cell migration of A549 and HeLa cells.