广州市科信局市科研条件建设（编号：穗科条[2009]5-11，穗科条[2013]277-1）；广州市科信局生物产业重大专项（编号：201300000053）。
目的 对比传统安全药理研究方法，论证遥测方法的优势。方法 分别使用传统安全药理方法和遥测技术对Beagle犬进行连续的心率、呼吸频率、血压和心电等生理指征监测，分析对比麻醉状态、清醒状态和采食前后Beagle犬的生理指标的变化。结果 维持麻醉会造成动物的心率、呼吸频率、血压和心电QT间期等出现显著变化；清醒动物24 h的生理指标变化不明显，并呈现出一定的生物节律；相对清醒动物，麻醉动物的心率显著升高、血压显著升高、心电QRS（QRS段）和QTcf（QTcf间期）显著延长、呼吸频率降低；采食后动物心率显著升高，QTcf极显著延长。结论 传统安全药理研究方法会影响动物心率、血压、呼吸频率和QT间期等生理指标，影响药物评价的客观性，通过遥测技术使用清醒动物进行安全药理研究能够减少误差，但在具体应用时需尽量排除外界干扰。
Objective To prove the advantages of telemetry by comparing with the traditional methods in safety pharmacology. Methods To monitor continuously the heart rate, respiratory rate, blood pressure and ECG of Beagle dogs by traditional and telemetry methods respectively, analyze and compare the changes between anesthetized and conscious dogs before and after feeding. Results Maintenance of anesthesia changed significantly the heart rate, respiratory rate, blood pressure and QT interval in the ECG of animals. The changes of physiological indicators in 24 h is not obvious in conscious animals, and showed a certain biorhythm. Compared with the conscious animals, the anesthetized dogs' heart rate was significantly higher, blood pressure increased significantly, QRS and QTcf interval prolonged significantly, respiratory frequency decreased, heart rate increased significantly after feeding, and QTcf interval extended very significantly. Conclusions Traditional methods in safety pharmacology affect animal physiological indicators such as heart rate, blood pressure, respiratory rate and QT interval, which affect the objectivity of drug evaluation. Using conscious animals by telemetry can reduce these errors, however, the interference from outside should be eliminated.
孙晶晶,周泉,齐珍珍,郭秋平.遥测方法对比传统安全药理学方法的优势及应用[J].中国比较医学杂志,2016,26(8):47~52.
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