Objective To study the anti-tumor and immune effects of FZHJF on H22 liver cancer-bearing mice.Methods The H22 transplanted mouse models were established and randomly divided into five groups:model group,low-,medium-and high-dose FZHJF(4.55,13.65,40.95g/kg)and 5-Fu groups(0.2mL/10g).12 days after treatment,the tumor inhibitory rate,liver index,thymus index,spleen index were calculated.At the same time,the appearance of liver tumor and tumor pathology in different groups was analyzed.Results Pathology results confirmed the tumors were hepatocelluar carcinoma.Tumor weight in FZHJF groups were lower than those of the model group(P<0.05);the tumor inhibitory rate increased with the dosage,and the tumor inhibitory rate in high dose group(49.02%)closed to 5-Fu group(51.51%);the results of tumor appearance showed that the tumor volume have the trend of reduce gradually,and is smaller than the control group.Thymus index and spleen index in FZHJF groups were markedly higher than those of the model and 5-FU groups(P<0.05).Conclusion FZHJF has significant anti-tumor effect on transplanted H22 tumor in mice and has the function of immunological enhancement,and these results will provide theoretical foundation for clinical practice of FZHJF.