Objective To establish formaldehyde-induced sinoatrial node damage model in rabbit.Methods 40% formaldehyde were used to damage the sinoatrial node area of rabbits. The parameters including heart rate, sinoatrial conduction time, sinus node recovery time, and the corrected sinus node recovery time were measured after formaldehyde-indeced damage. The diferences of the methods, degrees and duration of formaldehyde-indeced damage were compared, and the reabilities of models were confirmed by positive control drug in the experiments.Results(1) Damage method:In formaldehyde wet compressing method, the damage degree can be controled by regulating the compressing timing and area. Comparing to the former, the less injuries was caused and the damage degree could be controlled by regulating the drip speed of formaldehyde in the formaldehyde drip method; but it tends to injure the surrounding tissue of the sinus node.(2) Damage degree and mantainance of the model:the damages only maintained less than 7 days, when the percentage of heart rate decreased less than 30% of basic heart rate, and its lasted more than 70 days, when the percentage of heart rate decreased to 30%~60% of basic heart rate, but the heart rate decreased more than 60% of basic heart rate would lead to higher death rate.(3) Verification of positive drugs:both atropine and xinbao pill could increase the heart rates of sinoatrial node damage model, and improve the sinus function.Conclusions Formaldehyde wet compressing method has more reabilites than formaldehyde drip method, and the percentage decreased in heart rate of damage degree should be controlled between 30% to 60% of basic heart rate, so that the duration of models are longer, and the death rates are lower.