Objective To investigate the pathogenic dermal fungi in tree shrews from the center of germplasm resources, to provide the basic data for the experimental tree shrews' microbiological quality control.Methods 79 samples obtained from tree shrews were inoculated on Sabouraud's platiculture and slant cultivation,51 of which are from the wild origin and 28 from artificial feeding F1 generation, and they were subjected to microscope observation. Then extracted DNA with traditional methods. Amplify the ITS gene by PCR. Finally sequencing by blast of nucleotide homology analysis.Results 9 samples of the wild tree shrews turned into growing fungus, and so as 3 of the F1 generation. The carrying rate are 15.69% and 10.71% respectively. All fungi were found to be non-pathogenic followed BLAST. Conclusion The carrying rate of dermatophyte in artificial feeding of tree shrews is very low, and which decreased with the increasing breeding generation. Pathogenic dermal fungi has not been found.