Objective To understand the fat cells morphological changes during different periods under cold acclimation in tree shrews, whether the cold acclimation can induce cream-colored fat cells in white adipose tissue(beige adipocytes), and provide morphological basis.Methods The Hematoxylin-Eosin staining sections, Immunohistochemical staining sections and Immunofluorescence staining sections are compared.Results With the prolonged cold acclimation, the white fat cells and brown fat cells are decreased significantly, and the uncoupling protein1 expression in brown adipose tissues are increased. At the same time, abdominal subcutaneous white adipose tissue may have occurred browning, produce some shallow brown fat cells with UCP1 expression, namely the beige fat cells.Conclusions Different staining sections not only can clearly observe the morphological changes of fat cells, but also observe UCP1 from antibody positive color expression changes, and provide basic materials for the study of the adipose tissues in tree shrews.