Objective To establish a stable and simple method of tracheal intubation in rodents and to establish various models that needs of endotracheal intubation. Method Sixty Kunming mice anesthetized by midazlam and ketamin, were intubated by 22 GA arterial puncture needle casing with optical fiber. Then connect the casing and ventilator and make sure the casing has been into the trachea. Result 60 mice were all successfully intubated by casing with optical fiber with no throat mucosa bleeding, edema. Glottis and trachea don't present complications such as tracheal stenosis 7d later. Conclusion Fiber mediated intubation is a simple and useful method. It has a success rate with low incidence of complications.