Objective To observe the protective effect of Danggui Buxue Decoction on ⁶⁰Co-γ ray irradiated mice. Methods The experimental mice were randomly divided into 5 groups(the normal control group, model group, Danggui Buxue Decoction of low, middle and high dose groups). The mouse except the normal control group were irradiated by ⁶⁰Co-γ ray and established the radiation-damage model. Peripheral blood neutrophil count(NEUT), the number of bone marrow nucleated cells(BMC), the activity of serum superoxide dismutase(SOD) and the serum levels of malondialdehyde(MDA) were measured. Results Radiation injury in mice of NEUT, BMC and SOD were significantly decreased(P<0.01), MDA were increased(P<0.01). Danggui Buxue Decoction can increase the levels of NEUT, BMC and SOD in mice after radiation injury, decrease the content of MDA. And protective effect of high dose group was better(P<0.01). Conclusions Danggui Buxue Decoction has protective effect on radiation injury in mice. And there is a correlation between the protection effect and dose.