Objective To establish calcitonin gene-related peptide α(CGRPα) and β(CGRPβ) transgenic mice and analyze dynamic changes of blood pressure using this transgenic mouse model in hypertension research. Methods The transgenic vector was constructed by inserting the human CGRPα and CGRPβ gene into the down steam of chicken β-actin promoter, respectively. The transgenic mice were created by microinjection. The genotype of transgenic line was identified by PCR and the expression level of target gene was determined by Western blot. Dynamic changes of blood pressure was measured by noninvasive blood pressure meter. Results One line of human CGRPα C57BL/6J transgenic mice with high levels of CGRPα expression in the heart, lung, kidney and liver tissues was established. One line of human CGRPβ C57BL/6J transgenic mice with high levels of CGRPβ expression in the lung and liver tissues was established. Mice overexpressing the human CGRPα were normotensive, whereas mice overexpressing the human CGRPβ were hypotensive at 12 months of age. Conclusions CGRPα and CGRPβ transgenic mice may serve as an animal model for the mechanism research on the roles of CGRP on peripheral vascular resistance and hypertension resistance.