Objective The aim of this study was to provide a better positive control for allergic test by comparing the allergic effect of two kinds of positive materials, human albumin and ovalbumin, on active systemic anaphylaxis in guinea pig. Methods Guinea pigs were randomly divided into 14 groups, and were given human albumin, ovalbumin (2, 10, 100 mg/animal), or 0.9% sodium chloride injection as test substances, to assess the symptoms and incidence of systemic allergic responses induced by different sensitizing substances in different challenge doses and different challenge intervals.Results In the range of 2 to 100 mg/animal, the guinea pigs showed a 100% incidence rate of positive allergic reaction to human albumin and ovalbumin, the severity of anaphylactic symptoms was increasing along with the increase of sensitizing doses and challenge doses, and the allergic reaction was more strong induced by the same dose of ovalbumin than human albumin. Conclusions Our findings indicate that in the active systemic anaphylaxis test in guinea pigs, we recommend ovalbumin as the positive control in a dose of 2 mg/animal.