“十二五-艾滋病和病毒性肝炎等重大传染病防治”国家科技重大专项子课题(2012ZX10004501)。
ZHAN Ling-jun
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health;Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine;Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Pan Jia Yuan Nan Li No. 5, Chao Yang District, Beijing 100021, ChinaTANG Jun
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health;Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine;Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Pan Jia Yuan Nan Li No. 5, Chao Yang District, Beijing 100021, ChinaQIN Chuan
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health;Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine;Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Pan Jia Yuan Nan Li No. 5, Chao Yang District, Beijing 100021, ChinaPD-1:PD-L1/PD-L2通路在肿瘤和某些感染性疾病中发挥免疫抑制作用,而在结核病中的免疫作用尚不明确。结核菌感染后,免疫细胞上PD-1:PD-L1/PD-L2通路高表达,体外实验显示:用该通路的抗体阻断后保护性T细胞增强,而体内免疫作用不明确。 PD-1与配体PD-L1结合后可能发挥免疫抑制作用,其中 PD-L1表达受Th1细胞因子调控;PD-1与配体PD-L2结合后可能发挥免疫保护作用,其中PD-L2表达受Th2型细胞因子调控。利用Cas9-CRISPR基因敲除方法分别敲除特定免疫细胞上PD-1、PD-L1和PD-L2, 可研究PD-1、PD-L1和PD-L2在结核感染中免疫作用及机制,为结核病的防控提供理论依据。
PD-1:PD-L1/PD-L2 pathway played negative immune role in most tumor and several infectious diseases, but the immune role in tuberculosis (TB) was not explicited. Since the high expression of PD-1:PD-L1/PD-L2 pathway was correlated with MTB infection both in vitro and in vivo, the in vitro negative immune role was indicated by inhibited T cell protective immune function, while the in vivo immune role was not clear. The binding of PD-1 and PD-L1 played an immunosuppression role, the expression of PD-L1 was regulated by Th1-type cytokines. The binding of PD-1 and PD-L2 played protective immune role, and the expression was regulated by Th1-type cytokines. The immune role and mechanism of PD-1, PD-L1 and PD-L2 would be investigated by Cas9-CRISPR technique, which would provide theoretical basis for the tuberculosis prevention and control.
占玲俊,唐军,秦川. PD-1:PD-L1/PD-L2通路在结核感染中的免疫作用[J].中国比较医学杂志,2015,25(7):74~76.
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