脂肪干细胞分离纯化方法研究进展

doi:10.3969.j.issn.1671.7856. 2015.007.015

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脂肪干细胞分离纯化方法研究进展
DOI:
                        10.3969.j.issn.1671.7856. 2015.007.015
                    
作者:
                        刘琴刘琴
解放军广州军区武汉总医院医学实验科, 武汉 430070
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王丽平王丽平
解放军广州军区武汉总医院医学实验科, 武汉 430070
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陈芳陈芳
解放军广州军区武汉总医院医学实验科, 武汉 430070
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张宜张宜
解放军广州军区武汉总医院医学实验科, 武汉 430070
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Progress in the isolation and purification methods of adipose-derived stem cells

Author:

LIU Qin
LIU Qin
Department of Medical Experiments, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China
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WANG Li-ping
WANG Li-ping
Department of Medical Experiments, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China
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CHEN Fang
CHEN Fang
Department of Medical Experiments, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China
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ZHANG Yi
ZHANG Yi
Department of Medical Experiments, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China
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摘要:

脂肪干细胞作为种子细胞广泛应用于组织工程中,而获得足够量的、活性高的、高纯度的脂肪干细胞是其在组织工程中应用的前提。本文综述近几年来脂肪干细胞分离纯化方法的研究进展,比较各方法的优缺点,为分离纯化出理想的脂肪干细胞提供理论依据。

关键词:脂肪干细胞;分离;纯化;组织块贴壁法;酶消化法;免疫磁珠分选法;密度梯度离心法

Abstract:

Adipose-derived stem cells(ASCs) as potential seeded cells have been widely used in tissue engineering. Thus to obtain enough, high activity, high purity adipose-derived stem cells is the particular important premise of the application in tissue engineering. In this paper, the isolation and purification methods of ASCs were reviewed and the merit and demerit of different methods were compared in order to provide theoretical basis for safe and high-effective isolation and purification of ASCs.

Key words:Adipose-derived stem cells;Isolation Purification;Explants culture method;Enzymatic digestion method;Immunomagnetic beads sorting method;Density gradient centrifugation

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引用本文

刘琴,王丽平,陈芳,张宜.脂肪干细胞分离纯化方法研究进展[J].中国比较医学杂志,2015,25(7):69~73.

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最后修改日期:2015-06-10
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在线发布日期: 2015-07-28
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