目的 探讨制作实验小鼠骨髓病理切片的最佳取材部位。 方法 将实验小鼠颈椎脱臼处死后取胫骨、股骨、颅骨、胸骨、髂骨等5个不同部位制作骨髓病理切片,光镜下观察并拍照。比较不同部位骨髓实质及造血微环境的分布情况,选择最能反映骨髓造血功能的取材部位。 结果 髂骨骨髓切片可见大量分布均匀的红系、粒系细胞,血窦和巨核细胞清晰,支架组织结构紧密,脂肪细胞少。颅骨、胸骨、胫骨、股骨骨髓切片造血细胞显示不清,增生不活跃,且存在较多脂肪细胞。 结论 髂骨是作为制作骨髓切片的最佳取材部位之一。
Objective To explore the best sampling position of bone marrow biopsy in mice. Methods Mice were sacrificed by cervical dislocation, then take the skull, sternum, tibial, femoral and iliac bone, making pathological section. Observed and photographed under light microscope. Comparison of the distribution of the essence and the hematopoietic microenvironment in different parts. Select site which can best reflect the hematopoietic function of bone marrow. Results A large number of hematopoietic cells in ilium marrow sections visible. The cells are evenly distributed. Blood and megakaryocytes were clearly visible. The arrangement of the structure of the scaffolds for tissue closely. The number of fat cells less. Bone marrow hematopoietic cells in the skull, sternum, tibial, femoral were not clear and not active. And there are more fat cells. Conclusions As the best sampling position of bone marrow biopsy, is ilium.
冯璟,于远望.实验小鼠不同部位骨髓病理切片的比较[J].中国比较医学杂志,2015,25(7):64~65.
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