Objective To optimize the scheme of Mongolian gerbil superovulation. Methods Based on the analysis of the animal-week-old, the dose and time interval of hormone, we got the best animal-week-old, dose and time interval of hormone for Mongolian gerbil superovulation. Results The 6 week old female Mongolian gerbils which injected of 10 IU PMSG and followed by 10 IU hCG in 70 hours later could get the best superovulation. We collected eggs at 16 hour after mate with male gerbils. The ovum pick-up rate reached 80%, the number of oocytes were 32.6±3.0, the number of the fertilized egg developed to 2-cell are 24.8±5.4. Conclusions This study summarizes the optimization scheme of Mongolian gerbil superovulation induced by PMSG and hCG and it supported the foundation for Mongolian gerbils embryo biotechnology.