Objective To provide a theoretical basis for the prevention of Ebola virus disease, this survey aims to understand the distribution and prevalence status of Reston subtype of Ebola virus in north china area. Methods Collecting a variety of mammalian serums from zoos and farms in north china area, and detecting Ebola virus of Reston subtype. Results 109 kinds of collected mammalian serums, there were no positive samples detected by the Real-time PCR method. Conclusion Ebola virus has not been found in north china area, we should strengthen the publicity of Ebola virus disease, and do well security work.